Single-Molecule Analysis of Dynein Processivity and Stepping Behavior

Samara L. Reck-Peterson, Ahmet Yildiz, Andrew P. Carter, Arne Gennerich, Nan Zhang, Ronald D. Vale

Research output: Contribution to journalArticle

394 Citations (Scopus)

Abstract

Cytoplasmic dynein, the 1.2 MDa motor driving minus-end-directed motility, has been reported to move processively along microtubules, but its mechanism of motility remains poorly understood. Here, using S. cerevisiae to produce recombinant dynein with a chemically controlled dimerization switch, we show by structural and single-molecule analysis that processivity requires two dynein motor domains but not dynein's tail domain or any associated subunits. Dynein advances most frequently in 8 nm steps, although longer as well as side and backward steps are observed. Individual motor domains show a different stepping pattern, which is best explained by the two motor domains shuffling in an alternating manner between rear and forward positions. Our results suggest that cytoplasmic dynein moves processively through the coordination of its two motor domains, but its variable step size and direction suggest a considerable diffusional component to its step, which differs from Kinesin-1 and is more akin to myosin VI.

Original languageEnglish (US)
Pages (from-to)335-348
Number of pages14
JournalCell
Volume126
Issue number2
DOIs
StatePublished - Jul 28 2006
Externally publishedYes

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Dyneins
Cytoplasmic Dyneins
Molecules
Kinesin
Dimerization
Microtubules
Saccharomyces cerevisiae
Switches

ASJC Scopus subject areas

  • Cell Biology
  • Molecular Biology

Cite this

Reck-Peterson, S. L., Yildiz, A., Carter, A. P., Gennerich, A., Zhang, N., & Vale, R. D. (2006). Single-Molecule Analysis of Dynein Processivity and Stepping Behavior. Cell, 126(2), 335-348. https://doi.org/10.1016/j.cell.2006.05.046

Single-Molecule Analysis of Dynein Processivity and Stepping Behavior. / Reck-Peterson, Samara L.; Yildiz, Ahmet; Carter, Andrew P.; Gennerich, Arne; Zhang, Nan; Vale, Ronald D.

In: Cell, Vol. 126, No. 2, 28.07.2006, p. 335-348.

Research output: Contribution to journalArticle

Reck-Peterson, SL, Yildiz, A, Carter, AP, Gennerich, A, Zhang, N & Vale, RD 2006, 'Single-Molecule Analysis of Dynein Processivity and Stepping Behavior', Cell, vol. 126, no. 2, pp. 335-348. https://doi.org/10.1016/j.cell.2006.05.046
Reck-Peterson SL, Yildiz A, Carter AP, Gennerich A, Zhang N, Vale RD. Single-Molecule Analysis of Dynein Processivity and Stepping Behavior. Cell. 2006 Jul 28;126(2):335-348. https://doi.org/10.1016/j.cell.2006.05.046
Reck-Peterson, Samara L. ; Yildiz, Ahmet ; Carter, Andrew P. ; Gennerich, Arne ; Zhang, Nan ; Vale, Ronald D. / Single-Molecule Analysis of Dynein Processivity and Stepping Behavior. In: Cell. 2006 ; Vol. 126, No. 2. pp. 335-348.
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