Single-cell, locus-specific bisulfite sequencing (SLBS) for direct detection of epimutations in DNA methylation patterns

Silvia Gravina, Shireen Ganapathi, Jan Vijg

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

Stochastic epigenetic changes drive biological processes, such as development, aging and disease. Yet, epigenetic information is typically collected from millions of cells, thereby precluding a more precise understanding of cell-to-cell variability and the pathogenic history of epimutations. Here we present a novel procedure for directly detecting epimutations in DNA methylation patterns using single-cell, locus-specific bisulfite sequencing (SLBS). We show that within gene promoter regions of mouse hepatocytes the epimutation rate is two orders of magnitude higher than the mutation rate.

Original languageEnglish (US)
Pages (from-to)e93
JournalNucleic Acids Research
Volume43
Issue number14
DOIs
StatePublished - Aug 18 2015

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DNA Methylation
Epigenomics
Biological Phenomena
Mutation Rate
Genetic Promoter Regions
Hepatocytes
History
hydrogen sulfite
Genes

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Single-cell, locus-specific bisulfite sequencing (SLBS) for direct detection of epimutations in DNA methylation patterns. / Gravina, Silvia; Ganapathi, Shireen; Vijg, Jan.

In: Nucleic Acids Research, Vol. 43, No. 14, 18.08.2015, p. e93.

Research output: Contribution to journalArticle

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