Single-cell gene expression analysis of cholinergic neurons in the arcuate nucleus of the hypothalamus

Jae Hoon Jeong, Young JaeWoo, Streamson C. Chua, Jr., Young-Hwan Jo

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

The cholinoceptive system in the hypothalamus, in particular in the arcuate nucleus (ARC), plays a role in regulating food intake. Neurons in the ARC containmultiple neuropeptides, amines, and neurotransmitters. To study molecular and neurochemical heterogeneity of ARC neurons, we combine single-cell qRT-PCR and single-cell whole transcriptome amplification methods to analyze expression patterns of our hand-picked 60 genes in individual neurons in the ARC. Immunohistochemical and single-cell qRT-PCR analyses show choline acetyltransferase (ChAT)-expressing neurons in the ARC. Gene expression patterns are remarkably distinct in each individual cholinergic neuron. Two-thirds of cholinergic neurons express tyrosine hydroxylase (Th) mRNA. A large subset of these Th-positive cholinergic neurons is GABAergic as they express the GABA synthesizing enzyme glutamate decarboxylase and vesicular GABA transporter transcripts. Some cholinergic neurons also express the vesicular glutamate transporter transcript gene. POMC and POMC-processing enzyme transcripts are found in a subpopulation of cholinergic neurons. Despite this heterogeneity, gene expression patterns in individual cholinergic cells appear to be highly regulated in a cell-specific manner. In fact, membrane receptor transcripts are clustered with their respective intracellular signaling and downstream targets. This novel population of cholinergic neurons may be part of the neural circuitries that detect homeostatic need for food and control the drive to eat.

Original languageEnglish (US)
Article numbere0162839
JournalPLoS One
Volume11
Issue number9
DOIs
StatePublished - Sep 1 2016

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Arcuate Nucleus of Hypothalamus
Cholinergic Neurons
cholinergic agents
hypothalamus
Gene expression
Cholinergic Agents
Neurons
neurons
Gene Expression
gene expression
Tyrosine 3-Monooxygenase
cells
tyrosine 3-monooxygenase
Vesicular Glutamate Transport Proteins
Pro-Opiomelanocortin
Proprotein Convertases
gamma-aminobutyric acid
Polymerase Chain Reaction
Glutamate Decarboxylase
Choline O-Acetyltransferase

ASJC Scopus subject areas

  • Medicine(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

Single-cell gene expression analysis of cholinergic neurons in the arcuate nucleus of the hypothalamus. / Jeong, Jae Hoon; JaeWoo, Young; Chua, Jr., Streamson C.; Jo, Young-Hwan.

In: PLoS One, Vol. 11, No. 9, e0162839, 01.09.2016.

Research output: Contribution to journalArticle

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