Single cell behavior in metastatic primary mammary tumors correlated with gene expression patterns revealed by molecular profiling

Weigang Wang, Jeffrey B. Wyckoff, Victoria Centonze Frohlich, Yuri Oleynikov, Stefan Hüttelmaier, Jiri Zavadil, Lukas Cermak, Erwin P. Bottinger, Robert H. Singer, John G. White, Jeffrey E. Segall, John S. Condeelis

Research output: Contribution to journalArticle

326 Citations (Scopus)

Abstract

We have developed animal models of breast cancer that allow the direct examination of the behavior of individual green fluorescent protein-expressing carcinoma cells in live nonmetastatic and metastatic primary tumors in situ. We have combined this model with multiphoton microscopy to image differences in cell behavior within the primary tumor. Differences in cell behavior between nonmetastatic and metastatic cells in culture and within live primary tumors were correlated with results from cDNA microarray analyses to identify potentially important genetic determinants for breast cancer invasion and metastasis. Using multiphoton microscopy, we found five major differences in carcinoma cell behavior between the nonmetastatic and metastatic primary breast tumors involving extracellular matrix, cell motility, and chemotaxis. Behavioral differences were correlated with seven categories of molecules that were differentially expressed and related to these behaviors. We have found that extracellular matrix composition, actin nucleation factors, molecules involved in mechanical stability and survival, and cell polarity and chemotaxis showed large and consistent differences in gene expression. We conclude that aligning cell behavior in vivo with patterns of gene expression can lead to new insights into the microenvironment of carcinoma cells in the primary tumor and the molecular mechanisms behind cell behavior.

Original languageEnglish (US)
Pages (from-to)6278-6288
Number of pages11
JournalCancer Research
Volume62
Issue number21
StatePublished - Nov 1 2002

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Breast Neoplasms
Gene Expression
Chemotaxis
Carcinoma
Extracellular Matrix
Microscopy
Neoplasms
Cellular Microenvironment
Cell Polarity
Microarray Analysis
Green Fluorescent Proteins
Oligonucleotide Array Sequence Analysis
Cell Movement
Actins
Animal Models
Cell Culture Techniques
Neoplasm Metastasis

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Wang, W., Wyckoff, J. B., Frohlich, V. C., Oleynikov, Y., Hüttelmaier, S., Zavadil, J., ... Condeelis, J. S. (2002). Single cell behavior in metastatic primary mammary tumors correlated with gene expression patterns revealed by molecular profiling. Cancer Research, 62(21), 6278-6288.

Single cell behavior in metastatic primary mammary tumors correlated with gene expression patterns revealed by molecular profiling. / Wang, Weigang; Wyckoff, Jeffrey B.; Frohlich, Victoria Centonze; Oleynikov, Yuri; Hüttelmaier, Stefan; Zavadil, Jiri; Cermak, Lukas; Bottinger, Erwin P.; Singer, Robert H.; White, John G.; Segall, Jeffrey E.; Condeelis, John S.

In: Cancer Research, Vol. 62, No. 21, 01.11.2002, p. 6278-6288.

Research output: Contribution to journalArticle

Wang, W, Wyckoff, JB, Frohlich, VC, Oleynikov, Y, Hüttelmaier, S, Zavadil, J, Cermak, L, Bottinger, EP, Singer, RH, White, JG, Segall, JE & Condeelis, JS 2002, 'Single cell behavior in metastatic primary mammary tumors correlated with gene expression patterns revealed by molecular profiling', Cancer Research, vol. 62, no. 21, pp. 6278-6288.
Wang W, Wyckoff JB, Frohlich VC, Oleynikov Y, Hüttelmaier S, Zavadil J et al. Single cell behavior in metastatic primary mammary tumors correlated with gene expression patterns revealed by molecular profiling. Cancer Research. 2002 Nov 1;62(21):6278-6288.
Wang, Weigang ; Wyckoff, Jeffrey B. ; Frohlich, Victoria Centonze ; Oleynikov, Yuri ; Hüttelmaier, Stefan ; Zavadil, Jiri ; Cermak, Lukas ; Bottinger, Erwin P. ; Singer, Robert H. ; White, John G. ; Segall, Jeffrey E. ; Condeelis, John S. / Single cell behavior in metastatic primary mammary tumors correlated with gene expression patterns revealed by molecular profiling. In: Cancer Research. 2002 ; Vol. 62, No. 21. pp. 6278-6288.
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