Simultaneous use of standard and low-melting agarose for the separation and isolation of DNA by electrophoresis

U. Fotadar; L. E. Shapiro; M. I. Surks

Simultaneous use of standard and low-melting agarose for the separation and isolation of DNA by electrophoresis

U. Fotadar, L. E. Shapiro, M. I. Surks

Medicine

Research output: Contribution to journal › Article › peer-review

2 Scopus citations

Abstract

In this communication we describe the sequential use of standard and low-melting agarose in a single gel slab for the electrophoresis of DNA. This method has the advantages of high resolution and reproducibility characteristic of standard agarose and the ease of manipulation of DNA for direct cloning, sequential digestion and isolation, characteristic of low-melting agarose.

Original language	English (US)
Pages (from-to)	171-172
Number of pages	2
Journal	BioTechniques
Volume	10
Issue number	2
State	Published - 1991

ASJC Scopus subject areas

Biotechnology
General Biochemistry, Genetics and Molecular Biology

Cite this

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title = "Simultaneous use of standard and low-melting agarose for the separation and isolation of DNA by electrophoresis",

abstract = "In this communication we describe the sequential use of standard and low-melting agarose in a single gel slab for the electrophoresis of DNA. This method has the advantages of high resolution and reproducibility characteristic of standard agarose and the ease of manipulation of DNA for direct cloning, sequential digestion and isolation, characteristic of low-melting agarose.",

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AU - Fotadar, U.

AU - Shapiro, L. E.

AU - Surks, M. I.

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Y1 - 1991

N2 - In this communication we describe the sequential use of standard and low-melting agarose in a single gel slab for the electrophoresis of DNA. This method has the advantages of high resolution and reproducibility characteristic of standard agarose and the ease of manipulation of DNA for direct cloning, sequential digestion and isolation, characteristic of low-melting agarose.

AB - In this communication we describe the sequential use of standard and low-melting agarose in a single gel slab for the electrophoresis of DNA. This method has the advantages of high resolution and reproducibility characteristic of standard agarose and the ease of manipulation of DNA for direct cloning, sequential digestion and isolation, characteristic of low-melting agarose.

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JO - BioTechniques

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ER -

Simultaneous use of standard and low-melting agarose for the separation and isolation of DNA by electrophoresis

Abstract

ASJC Scopus subject areas

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