@article{5d3890da14bb443e9379a8342f66c70b,
title = "Simultaneous two-photon calcium imaging at different depths with spatiotemporal multiplexing",
abstract = "In vivo two-photon calcium imaging would benefit from the use of multiple excitation beams to increase scanning speed, signal-to-noise ratio and field of view or to image different axial planes simultaneously. Using spatiotemporal multiplexing we circumvented light-scattering ambiguity inherent to deep-tissue multifocal two-photon microscopy. We demonstrate calcium imaging at multiple axial planes in the intact mouse brain to monitor network activity of ensembles of cortical neurons in three spatial dimensions.",
author = "Adrian Cheng and Gon{\c c}alves, {J. Tiago} and Peyman Golshani and Katsushi Arisaka and Carlos Portera-Cailliau",
note = "Funding Information: We thank D. Kleinfeld, J.F. L{\'e}ger, T. Otis and members of the Portera-Cailliau laboratory for discussions and comments on the manuscript, and M. Suyama and Y. Kawai (Hamamatsu Photonics K.K.) for engineering support. This work was supported by grants from the US National Institutes of Health (the Eunice Kennedy Shriver National Institute of Child Health and Human Development, the National Institute of Neurological Disorders and Stroke) and the US National Science Foundation (Major Research Instrumentation Program).",
year = "2011",
month = feb,
doi = "10.1038/nmeth.1552",
language = "English (US)",
volume = "8",
pages = "139--142",
journal = "Nature Methods",
issn = "1548-7091",
publisher = "Nature Publishing Group",
number = "2",
}