Simultaneous two-photon calcium imaging at different depths with spatiotemporal multiplexing

Adrian Cheng, J. Tiago Goncalves, Peyman Golshani, Katsushi Arisaka, Carlos Portera-Cailliau

Research output: Contribution to journalArticle

159 Citations (Scopus)

Abstract

In vivo two-photon calcium imaging would benefit from the use of multiple excitation beams to increase scanning speed, signal-to-noise ratio and field of view or to image different axial planes simultaneously. Using spatiotemporal multiplexing we circumvented light-scattering ambiguity inherent to deep-tissue multifocal two-photon microscopy. We demonstrate calcium imaging at multiple axial planes in the intact mouse brain to monitor network activity of ensembles of cortical neurons in three spatial dimensions.

Original languageEnglish (US)
Pages (from-to)139-142
Number of pages4
JournalNature Methods
Volume8
Issue number2
DOIs
StatePublished - Feb 2011
Externally publishedYes

Fingerprint

Photons
Multiplexing
Calcium
Imaging techniques
Signal-To-Noise Ratio
Light scattering
Neurons
Microscopy
Brain
Signal to noise ratio
Microscopic examination
Tissue
Scanning
Light

ASJC Scopus subject areas

  • Biotechnology
  • Molecular Biology
  • Biochemistry
  • Cell Biology

Cite this

Simultaneous two-photon calcium imaging at different depths with spatiotemporal multiplexing. / Cheng, Adrian; Goncalves, J. Tiago; Golshani, Peyman; Arisaka, Katsushi; Portera-Cailliau, Carlos.

In: Nature Methods, Vol. 8, No. 2, 02.2011, p. 139-142.

Research output: Contribution to journalArticle

Cheng, Adrian ; Goncalves, J. Tiago ; Golshani, Peyman ; Arisaka, Katsushi ; Portera-Cailliau, Carlos. / Simultaneous two-photon calcium imaging at different depths with spatiotemporal multiplexing. In: Nature Methods. 2011 ; Vol. 8, No. 2. pp. 139-142.
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