Simultaneous exposure to vinylcyclohexene and methylmercury in Drosophila melanogaster: Biochemical and molecular analyses

Bruna Candia Piccoli, Ana Lúcia Anversa Segatto, Claúdia Sirlene Oliveira, Fernanda D'Avila Da Silva, Michael Aschner, Joaõ Batista Teixeira Da Rocha

Research output: Contribution to journalArticle

Abstract

Background: Exposure to vinylcyclohexene (VCH) and methylmercury (MeHg+) can induce oxidative stress and gene modulation. Several studies have been evaluating the effects of VCH and MeHg+, but little is known about interactive effects between them. This work aimed to assess the exposure and co-exposure effects of MeHg+ and VCH on oxidative stress and gene modulation in Drosophila melanogaster. Methods: Reactive species production, glutathione S-transferase (GST) and acetylcholinesterase (AChE) activities were evaluated after exposure and co-exposure to VCH (1 mM) and MeHg+ (0.2 mM) for one or three days in the head and body (thorax and abdomen) of flies. The expression of genes related to redox state and inflammatory response was evaluated after exposure and co-exposure to VCH and MeHg+ for three days. Results: Survival decreased only in flies co-exposed to VCH and MeHg+ for three days. All treatments increased total reactive species production after one day of exposure. However, no significant changes were observed in the head after three days of exposure. One day of exposure to VCH caused an increase in the head GST activity, whereas MeHg+ induced an increase after three days of exposure. Regarding the body, all treatments increased GST activity after one day of exposure, but only the flies exposed to MeHg+ presented an increase in GST activity after three days of exposure. Treatments did not alter AChE activity in the head. As for gene expression, there was a significant increase in the Relish transcription factor gene in the flies' body, but Nrf2, Keap1, Jafrac1, TrxR1, and NF-κβ were not altered. Conclusion: The results suggest that exposure to VCH and MeHg+ induce oxidative stress and activation of an inflammatory response in fruit flies.

Original languageEnglish (US)
Article number83
JournalBMC Pharmacology and Toxicology
Volume20
DOIs
StatePublished - Dec 19 2019

Fingerprint

Drosophila melanogaster
Diptera
Glutathione Transferase
Head
Oxidative Stress
Acetylcholinesterase
Genes
Gene Expression
Abdomen
Oxidation-Reduction
Fruit
Transcription Factors
Thorax

Keywords

  • Alternative model
  • Drosophila melanogaster
  • Inflammatory response
  • Oxidative stress
  • Xenobiotic

ASJC Scopus subject areas

  • Pharmacology
  • Pharmacology (medical)

Cite this

Simultaneous exposure to vinylcyclohexene and methylmercury in Drosophila melanogaster : Biochemical and molecular analyses. / Piccoli, Bruna Candia; Segatto, Ana Lúcia Anversa; Oliveira, Claúdia Sirlene; D'Avila Da Silva, Fernanda; Aschner, Michael; Da Rocha, Joaõ Batista Teixeira.

In: BMC Pharmacology and Toxicology, Vol. 20, 83, 19.12.2019.

Research output: Contribution to journalArticle

Piccoli, Bruna Candia ; Segatto, Ana Lúcia Anversa ; Oliveira, Claúdia Sirlene ; D'Avila Da Silva, Fernanda ; Aschner, Michael ; Da Rocha, Joaõ Batista Teixeira. / Simultaneous exposure to vinylcyclohexene and methylmercury in Drosophila melanogaster : Biochemical and molecular analyses. In: BMC Pharmacology and Toxicology. 2019 ; Vol. 20.
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abstract = "Background: Exposure to vinylcyclohexene (VCH) and methylmercury (MeHg+) can induce oxidative stress and gene modulation. Several studies have been evaluating the effects of VCH and MeHg+, but little is known about interactive effects between them. This work aimed to assess the exposure and co-exposure effects of MeHg+ and VCH on oxidative stress and gene modulation in Drosophila melanogaster. Methods: Reactive species production, glutathione S-transferase (GST) and acetylcholinesterase (AChE) activities were evaluated after exposure and co-exposure to VCH (1 mM) and MeHg+ (0.2 mM) for one or three days in the head and body (thorax and abdomen) of flies. The expression of genes related to redox state and inflammatory response was evaluated after exposure and co-exposure to VCH and MeHg+ for three days. Results: Survival decreased only in flies co-exposed to VCH and MeHg+ for three days. All treatments increased total reactive species production after one day of exposure. However, no significant changes were observed in the head after three days of exposure. One day of exposure to VCH caused an increase in the head GST activity, whereas MeHg+ induced an increase after three days of exposure. Regarding the body, all treatments increased GST activity after one day of exposure, but only the flies exposed to MeHg+ presented an increase in GST activity after three days of exposure. Treatments did not alter AChE activity in the head. As for gene expression, there was a significant increase in the Relish transcription factor gene in the flies' body, but Nrf2, Keap1, Jafrac1, TrxR1, and NF-κβ were not altered. Conclusion: The results suggest that exposure to VCH and MeHg+ induce oxidative stress and activation of an inflammatory response in fruit flies.",
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T1 - Simultaneous exposure to vinylcyclohexene and methylmercury in Drosophila melanogaster

T2 - Biochemical and molecular analyses

AU - Piccoli, Bruna Candia

AU - Segatto, Ana Lúcia Anversa

AU - Oliveira, Claúdia Sirlene

AU - D'Avila Da Silva, Fernanda

AU - Aschner, Michael

AU - Da Rocha, Joaõ Batista Teixeira

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AB - Background: Exposure to vinylcyclohexene (VCH) and methylmercury (MeHg+) can induce oxidative stress and gene modulation. Several studies have been evaluating the effects of VCH and MeHg+, but little is known about interactive effects between them. This work aimed to assess the exposure and co-exposure effects of MeHg+ and VCH on oxidative stress and gene modulation in Drosophila melanogaster. Methods: Reactive species production, glutathione S-transferase (GST) and acetylcholinesterase (AChE) activities were evaluated after exposure and co-exposure to VCH (1 mM) and MeHg+ (0.2 mM) for one or three days in the head and body (thorax and abdomen) of flies. The expression of genes related to redox state and inflammatory response was evaluated after exposure and co-exposure to VCH and MeHg+ for three days. Results: Survival decreased only in flies co-exposed to VCH and MeHg+ for three days. All treatments increased total reactive species production after one day of exposure. However, no significant changes were observed in the head after three days of exposure. One day of exposure to VCH caused an increase in the head GST activity, whereas MeHg+ induced an increase after three days of exposure. Regarding the body, all treatments increased GST activity after one day of exposure, but only the flies exposed to MeHg+ presented an increase in GST activity after three days of exposure. Treatments did not alter AChE activity in the head. As for gene expression, there was a significant increase in the Relish transcription factor gene in the flies' body, but Nrf2, Keap1, Jafrac1, TrxR1, and NF-κβ were not altered. Conclusion: The results suggest that exposure to VCH and MeHg+ induce oxidative stress and activation of an inflammatory response in fruit flies.

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KW - Inflammatory response

KW - Oxidative stress

KW - Xenobiotic

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