Short-term metabolic fate of L-[13N]glutamate in the Walker 256 carcinosarcoma in vivo

Sabina Filc-DeRicco, Alan S. Gelbard, Arthur J L Cooper, Karen C. Rosenspire, Edward Nieves

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

In vivo studies with L-[13N]glutamate in the Walker 256 carcinosarcoma implanted under the renal capsule of female Sprague-Dawley rats demonstrate that uptake of glutamate and the rate of incorporation of the nitrogen label from this amino acid into metabolites is slower in the tumor than in nontumorous kidney tissue. Glutamate dehydrogenase, glutaminase, and alanine aminotransferase activities are significantly lower within the tumor than within the adjoining kidney. However, the tumor expresses high levels of aspartate aminotransferase, attesting to the importance of this enzyme in the metabolism of glutamate. Indeed, high performance liquid chromatographic analysis showed that the principal metabolic fate of label derived from L-[13N]glutamate in the tumor is incorporation into aspartate. Measurement of specific activity ratios of glutamate to aspartate shows that the transfer, of nitrogen from glutamate to aspartate is rapid and that equilibration of label among components of the aspartate aminotransferase reaction is attained within minutes after tumor uptake. Analyses of the nontumorous portion of the implanted kidney also showed that aspartate is the major recipient of glutamate nitrogen. However, high performance liquid chromatographic analyses of deproteinized tissue revealed that glutamine and ammonia are also significant 13N-labeled metabolites formed from L-[13N]glutamate within the kidney. Proportionately lower amounts of these labeled metabolites were found in the tumor.

Original languageEnglish (US)
Pages (from-to)4839-4844
Number of pages6
JournalCancer Research
Volume50
Issue number16
StatePublished - Aug 15 1990
Externally publishedYes

Fingerprint

Walker Carcinoma 256
Glutamic Acid
Aspartic Acid
Kidney
Neoplasms
Nitrogen
Aspartate Aminotransferases
High Pressure Liquid Chromatography
Glutaminase
Glutamate Dehydrogenase
Glutamine
Alanine Transaminase
Ammonia
Capsules
Sprague Dawley Rats
Amino Acids

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Filc-DeRicco, S., Gelbard, A. S., Cooper, A. J. L., Rosenspire, K. C., & Nieves, E. (1990). Short-term metabolic fate of L-[13N]glutamate in the Walker 256 carcinosarcoma in vivo. Cancer Research, 50(16), 4839-4844.

Short-term metabolic fate of L-[13N]glutamate in the Walker 256 carcinosarcoma in vivo. / Filc-DeRicco, Sabina; Gelbard, Alan S.; Cooper, Arthur J L; Rosenspire, Karen C.; Nieves, Edward.

In: Cancer Research, Vol. 50, No. 16, 15.08.1990, p. 4839-4844.

Research output: Contribution to journalArticle

Filc-DeRicco, S, Gelbard, AS, Cooper, AJL, Rosenspire, KC & Nieves, E 1990, 'Short-term metabolic fate of L-[13N]glutamate in the Walker 256 carcinosarcoma in vivo', Cancer Research, vol. 50, no. 16, pp. 4839-4844.
Filc-DeRicco S, Gelbard AS, Cooper AJL, Rosenspire KC, Nieves E. Short-term metabolic fate of L-[13N]glutamate in the Walker 256 carcinosarcoma in vivo. Cancer Research. 1990 Aug 15;50(16):4839-4844.
Filc-DeRicco, Sabina ; Gelbard, Alan S. ; Cooper, Arthur J L ; Rosenspire, Karen C. ; Nieves, Edward. / Short-term metabolic fate of L-[13N]glutamate in the Walker 256 carcinosarcoma in vivo. In: Cancer Research. 1990 ; Vol. 50, No. 16. pp. 4839-4844.
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