Short-term in vitro sensitivity testing in acute leukemia.

J. D. Schwarzmeier, R. Pirker, Elisabeth M. Paietta

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

To detect sensitivity or resistance of leukemic cells to chemotherapy prior to treatment, a short-term incubation method was employed. Blast cells from the peripheral blood or bone marrow of adult patients with different forms of acute leukemia were analyzed for in vitro responsiveness to cytostatic agents in terms of suppression of nucleoside precursor (3H-uridine, 3H-deoxyuridine) incorporation into the cells. Retrospectively, the in vitro data were compared to the clinical response of the patients to polychemotherapy. In the majority of patients, in vitro cytotoxic effectiveness of doxorubicin and ara-C reflected the in vivo situation. The levels of in vitro inhibition that could distinguish between drug-sensitive and drug-resistant diseases appeared to be 30% for doxorubicin and 20% for ara-C. No correlation was found between the doxorubicin effect in vitro and the proliferative state of the leukemic cell populations. Serial in vitro testing during the course of the disease of various patients proved the ability of the test system to detect acquired resistance to chemotherapeutic agents. Studies with established cell lines (HL-60, Raji) indicated that the short-term test in its present form is probably not suited to monitor the effect of drug combinations in vitro.

Original languageEnglish (US)
Pages (from-to)116-126
Number of pages11
JournalRecent results in cancer research. Fortschritte der Krebsforschung. Progres dans les recherches sur le cancer
Volume94
StatePublished - 1984
Externally publishedYes

Fingerprint

Leukemia
Doxorubicin
Cytarabine
Deoxyuridine
Cytostatic Agents
Drug Combinations
In Vitro Techniques
Combination Drug Therapy
Nucleosides
Pharmaceutical Preparations
Blood Cells
Bone Marrow
Drug Therapy
Cell Line
Population

ASJC Scopus subject areas

  • Medicine(all)

Cite this

@article{b14ece72ceee4c119bfe44166fe91a44,
title = "Short-term in vitro sensitivity testing in acute leukemia.",
abstract = "To detect sensitivity or resistance of leukemic cells to chemotherapy prior to treatment, a short-term incubation method was employed. Blast cells from the peripheral blood or bone marrow of adult patients with different forms of acute leukemia were analyzed for in vitro responsiveness to cytostatic agents in terms of suppression of nucleoside precursor (3H-uridine, 3H-deoxyuridine) incorporation into the cells. Retrospectively, the in vitro data were compared to the clinical response of the patients to polychemotherapy. In the majority of patients, in vitro cytotoxic effectiveness of doxorubicin and ara-C reflected the in vivo situation. The levels of in vitro inhibition that could distinguish between drug-sensitive and drug-resistant diseases appeared to be 30{\%} for doxorubicin and 20{\%} for ara-C. No correlation was found between the doxorubicin effect in vitro and the proliferative state of the leukemic cell populations. Serial in vitro testing during the course of the disease of various patients proved the ability of the test system to detect acquired resistance to chemotherapeutic agents. Studies with established cell lines (HL-60, Raji) indicated that the short-term test in its present form is probably not suited to monitor the effect of drug combinations in vitro.",
author = "Schwarzmeier, {J. D.} and R. Pirker and Paietta, {Elisabeth M.}",
year = "1984",
language = "English (US)",
volume = "94",
pages = "116--126",
journal = "Recent Results in Cancer Research",
issn = "0080-0015",
publisher = "Springer New York",

}

TY - JOUR

T1 - Short-term in vitro sensitivity testing in acute leukemia.

AU - Schwarzmeier, J. D.

AU - Pirker, R.

AU - Paietta, Elisabeth M.

PY - 1984

Y1 - 1984

N2 - To detect sensitivity or resistance of leukemic cells to chemotherapy prior to treatment, a short-term incubation method was employed. Blast cells from the peripheral blood or bone marrow of adult patients with different forms of acute leukemia were analyzed for in vitro responsiveness to cytostatic agents in terms of suppression of nucleoside precursor (3H-uridine, 3H-deoxyuridine) incorporation into the cells. Retrospectively, the in vitro data were compared to the clinical response of the patients to polychemotherapy. In the majority of patients, in vitro cytotoxic effectiveness of doxorubicin and ara-C reflected the in vivo situation. The levels of in vitro inhibition that could distinguish between drug-sensitive and drug-resistant diseases appeared to be 30% for doxorubicin and 20% for ara-C. No correlation was found between the doxorubicin effect in vitro and the proliferative state of the leukemic cell populations. Serial in vitro testing during the course of the disease of various patients proved the ability of the test system to detect acquired resistance to chemotherapeutic agents. Studies with established cell lines (HL-60, Raji) indicated that the short-term test in its present form is probably not suited to monitor the effect of drug combinations in vitro.

AB - To detect sensitivity or resistance of leukemic cells to chemotherapy prior to treatment, a short-term incubation method was employed. Blast cells from the peripheral blood or bone marrow of adult patients with different forms of acute leukemia were analyzed for in vitro responsiveness to cytostatic agents in terms of suppression of nucleoside precursor (3H-uridine, 3H-deoxyuridine) incorporation into the cells. Retrospectively, the in vitro data were compared to the clinical response of the patients to polychemotherapy. In the majority of patients, in vitro cytotoxic effectiveness of doxorubicin and ara-C reflected the in vivo situation. The levels of in vitro inhibition that could distinguish between drug-sensitive and drug-resistant diseases appeared to be 30% for doxorubicin and 20% for ara-C. No correlation was found between the doxorubicin effect in vitro and the proliferative state of the leukemic cell populations. Serial in vitro testing during the course of the disease of various patients proved the ability of the test system to detect acquired resistance to chemotherapeutic agents. Studies with established cell lines (HL-60, Raji) indicated that the short-term test in its present form is probably not suited to monitor the effect of drug combinations in vitro.

UR - http://www.scopus.com/inward/record.url?scp=0021192396&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0021192396&partnerID=8YFLogxK

M3 - Article

C2 - 6593775

AN - SCOPUS:0021192396

VL - 94

SP - 116

EP - 126

JO - Recent Results in Cancer Research

JF - Recent Results in Cancer Research

SN - 0080-0015

ER -