Short report: A non-radioactive DAPI-based high-throughput in vitro assay to assess Plasmodium falciparum responsiveness to antimalarials - Increased sensitivity of P. falciparum to chloroquine in senegal

Daouda Ndiaye, Vishal Patel, Allison Demas, Michele LeRoux, Omar Ndir, Souleymane Mboup, Jon Clardy, Viswanathan Lakshmanan, Johanna P. Daily, Dyann F. Wirth

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

The spread of Plasmodium falciparum drug resistance is outpacing new antimalarial development and compromising effective malaria treatment. Combination therapy is widely implemented to prolong the effectiveness of currently approved antimalarials.To maximize utility of available drugs, periodic monitoring of drug efficacy and gathering of accurate information regarding parasite-sensitivity changes are essential. We describe a high-throughput, non-radioactive, field-based assay to evaluate in vitro antimalarial drug sensitivity of P. falciparum isolates from 40 Senegalese patients. Compared with earlier years, we found a significant decrease in chloroquine in vitro and in genotypic resistances (> 50% and > 65%, respectively, in previous studies) with only 23% of isolates showing resistance. This is possibly caused by a withdrawal of chloroquine from Senegal in 2002. We also found a range of artemisinin responses. Prevalence of drug resistance is dynamic and varies by region. Therefore, the implementation of non-radioactive, robust, high-throughput antimalarial sensitivity assays is critical for defining region-specific prophylaxis and treatment guidelines.

Original languageEnglish (US)
Pages (from-to)228-230
Number of pages3
JournalAmerican Journal of Tropical Medicine and Hygiene
Volume82
Issue number2
DOIs
StatePublished - Feb 2010

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Senegal
Chloroquine
Antimalarials
Plasmodium falciparum
Drug Resistance
Drug Monitoring
Malaria
Parasites
Therapeutics
Guidelines
DAPI
In Vitro Techniques
Pharmaceutical Preparations

ASJC Scopus subject areas

  • Parasitology
  • Infectious Diseases
  • Virology
  • Medicine(all)

Cite this

Short report : A non-radioactive DAPI-based high-throughput in vitro assay to assess Plasmodium falciparum responsiveness to antimalarials - Increased sensitivity of P. falciparum to chloroquine in senegal. / Ndiaye, Daouda; Patel, Vishal; Demas, Allison; LeRoux, Michele; Ndir, Omar; Mboup, Souleymane; Clardy, Jon; Lakshmanan, Viswanathan; Daily, Johanna P.; Wirth, Dyann F.

In: American Journal of Tropical Medicine and Hygiene, Vol. 82, No. 2, 02.2010, p. 228-230.

Research output: Contribution to journalArticle

Ndiaye, Daouda ; Patel, Vishal ; Demas, Allison ; LeRoux, Michele ; Ndir, Omar ; Mboup, Souleymane ; Clardy, Jon ; Lakshmanan, Viswanathan ; Daily, Johanna P. ; Wirth, Dyann F. / Short report : A non-radioactive DAPI-based high-throughput in vitro assay to assess Plasmodium falciparum responsiveness to antimalarials - Increased sensitivity of P. falciparum to chloroquine in senegal. In: American Journal of Tropical Medicine and Hygiene. 2010 ; Vol. 82, No. 2. pp. 228-230.
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