Shark, a Src homology 2, ankyrin repeat, tyrosine kinase, is expressed on the apical surfaces of ectodermal epithelia

Anthony W. Ferrante, Rosemary Reinke, E. Richard Stanley

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

Tyrosine kinases, ankyrin repeats, and Src homology 2 domains play central roles in developmental processes. The cloning of a cDNA for Shark, a single protein that possesses all three domains, is described. During Drosophila embryogenesis, Shark is expressed exclusively by ectodermally derived epithelia and is localized preferentially to the apical surface of these cells. This apical localization persists, even as tissues undergo complex invaginations, moving from the external surface of embryos to form internal structures, but expression is lost when cells lose their polarity. This pattern closely mimics the expression of Crumbs, a protein necessary for proper organization of ectodermal epithelia. Shark's structure and localization pattern suggest that it functions in a signaling pathway for epithelial cell polarity, possibly transducing the Crumbs signal.

Original languageEnglish (US)
Pages (from-to)1911-1915
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume92
Issue number6
DOIs
StatePublished - Mar 14 1995

Fingerprint

Ankyrin Repeat
Sharks
Protein-Tyrosine Kinases
Epithelium
Cell Polarity
src Homology Domains
Drosophila
Embryonic Development
Organism Cloning
Proteins
Embryonic Structures
Complementary DNA
Epithelial Cells

Keywords

  • Drosophila
  • invagination
  • signal transduction

ASJC Scopus subject areas

  • Genetics
  • General

Cite this

Shark, a Src homology 2, ankyrin repeat, tyrosine kinase, is expressed on the apical surfaces of ectodermal epithelia. / Ferrante, Anthony W.; Reinke, Rosemary; Stanley, E. Richard.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 92, No. 6, 14.03.1995, p. 1911-1915.

Research output: Contribution to journalArticle

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