SH2 domains recognize specific phosphopeptide sequences

Songyang Zhou, Steven E. Shoelson, Manas Chaudhuri, Gerald Gish, Tony Pawson, Wayne G. Haser, Fred King, Tom Roberts, Sheldon Ratnofsky, Robert J. Lechleider, Benjamin G. Neel, Raymond B. Birge, Jorge E. Fajardo, Margaret M. Chou, Hidesaburo Hanafusa, Brian Schaffhausen, Lewis C. Cantley

Research output: Contribution to journalArticle

2284 Citations (Scopus)

Abstract

A phosphopeptide library was used to determine the sequence specificity of the peptide-binding sites of SH2 domains. One group of SH2 domains (Src, Fyn, Lck, Fgr, Abl, Crk, and Nck) preferred sequences with the general motif pTyr-hydrophilic-hydrophilic-lle/Pro while another group (SH2 domains of p85, phosphollpase C-γ, and SHPTP2) selected the general motif pTyr-hydrophobic-X-hydrophobic. Individual members of these groups selected unique sequences, except the Src subfamily (Src, F́yn, Lck, and Fgr), which all selected the sequence pTyr-Glu-Glu-Ile. The variability in SH2 domain sequences at likely sites of contact provides a structural basis for the phosphopeptide selectivity of these families. Possible in vivo binding sites of the SH2 domains are discussed.

Original languageEnglish (US)
Pages (from-to)767-778
Number of pages12
JournalCell
Volume72
Issue number5
DOIs
StatePublished - Mar 12 1993
Externally publishedYes

Fingerprint

Phosphopeptides
src Homology Domains
Non-Receptor Type 11 Protein Tyrosine Phosphatase
Binding Sites
glutamyl-glutamic acid
Peptides

ASJC Scopus subject areas

  • Cell Biology
  • Molecular Biology

Cite this

Zhou, S., Shoelson, S. E., Chaudhuri, M., Gish, G., Pawson, T., Haser, W. G., ... Cantley, L. C. (1993). SH2 domains recognize specific phosphopeptide sequences. Cell, 72(5), 767-778. https://doi.org/10.1016/0092-8674(93)90404-E

SH2 domains recognize specific phosphopeptide sequences. / Zhou, Songyang; Shoelson, Steven E.; Chaudhuri, Manas; Gish, Gerald; Pawson, Tony; Haser, Wayne G.; King, Fred; Roberts, Tom; Ratnofsky, Sheldon; Lechleider, Robert J.; Neel, Benjamin G.; Birge, Raymond B.; Fajardo, Jorge E.; Chou, Margaret M.; Hanafusa, Hidesaburo; Schaffhausen, Brian; Cantley, Lewis C.

In: Cell, Vol. 72, No. 5, 12.03.1993, p. 767-778.

Research output: Contribution to journalArticle

Zhou, S, Shoelson, SE, Chaudhuri, M, Gish, G, Pawson, T, Haser, WG, King, F, Roberts, T, Ratnofsky, S, Lechleider, RJ, Neel, BG, Birge, RB, Fajardo, JE, Chou, MM, Hanafusa, H, Schaffhausen, B & Cantley, LC 1993, 'SH2 domains recognize specific phosphopeptide sequences', Cell, vol. 72, no. 5, pp. 767-778. https://doi.org/10.1016/0092-8674(93)90404-E
Zhou S, Shoelson SE, Chaudhuri M, Gish G, Pawson T, Haser WG et al. SH2 domains recognize specific phosphopeptide sequences. Cell. 1993 Mar 12;72(5):767-778. https://doi.org/10.1016/0092-8674(93)90404-E
Zhou, Songyang ; Shoelson, Steven E. ; Chaudhuri, Manas ; Gish, Gerald ; Pawson, Tony ; Haser, Wayne G. ; King, Fred ; Roberts, Tom ; Ratnofsky, Sheldon ; Lechleider, Robert J. ; Neel, Benjamin G. ; Birge, Raymond B. ; Fajardo, Jorge E. ; Chou, Margaret M. ; Hanafusa, Hidesaburo ; Schaffhausen, Brian ; Cantley, Lewis C. / SH2 domains recognize specific phosphopeptide sequences. In: Cell. 1993 ; Vol. 72, No. 5. pp. 767-778.
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