Sex-Specific Response of Caenorhabditis elegans to Methylmercury Toxicity

Joanna A. Ruszkiewicz, Gabriel Teixeira de Macedo, Antonio Miranda-Vizuete, Aaron B. Bowman, Julia Bornhorst, Tanja Schwerdtle, Felix A. Antunes Soares, Michael Aschner

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Methylmercury (MeHg), an abundant environmental pollutant, has long been known to adversely affect neurodevelopment in both animals and humans. Several reports from epidemiological studies, as well as experimental data indicate sex-specific susceptibility to this neurotoxicant; however, the molecular bases of this process are still not clear. In the present study, we used Caenorhabditis elegans (C. elegans), to investigate sex differences in response to MeHg toxicity during development. Worms at different developmental stage (L1, L4, and adult) were treated with MeHg for 1 h. Lethality assays revealed that male worms exhibited significantly higher resistance to MeHg than hermaphrodites, when at L4 stage or adults. However, the number of worms with degenerated neurons was unaffected by MeHg, both in males and hermaphrodites. Lower susceptibility of males was not related to changes in mercury (Hg) accumulation, which was analogous for both wild-type (wt) and male-rich him-8 strain. Total glutathione (GSH) levels decreased upon MeHg in him-8, but not in wt. Moreover, the sex-dependent response of the cytoplasmic thioredoxin system was observed—males exhibited significantly higher expression of thioredoxin TRX-1, and thioredoxin reductase TRXR-1 expression was downregulated upon MeHg treatment only in hermaphrodites. These outcomes indicate that the redox status is an important contributor to sex-specific sensitivity to MeHg in C. elegans.

Original languageEnglish (US)
JournalNeurotoxicity Research
DOIs
StateAccepted/In press - Jan 1 2018

Fingerprint

Thioredoxins
Caenorhabditis elegans
Toxicity
Thioredoxin Reductase 1
Environmental Pollutants
Mercury
Neurons
Glutathione
Assays
Animals
Sex Characteristics
Oxidation-Reduction
Epidemiologic Studies
Down-Regulation

Keywords

  • Antioxidant
  • C. elegans
  • Male
  • Methylmercury
  • Sex
  • Thioredoxin

ASJC Scopus subject areas

  • Neuroscience(all)
  • Toxicology

Cite this

Ruszkiewicz, J. A., Teixeira de Macedo, G., Miranda-Vizuete, A., Bowman, A. B., Bornhorst, J., Schwerdtle, T., ... Aschner, M. (Accepted/In press). Sex-Specific Response of Caenorhabditis elegans to Methylmercury Toxicity. Neurotoxicity Research. https://doi.org/10.1007/s12640-018-9949-4

Sex-Specific Response of Caenorhabditis elegans to Methylmercury Toxicity. / Ruszkiewicz, Joanna A.; Teixeira de Macedo, Gabriel; Miranda-Vizuete, Antonio; Bowman, Aaron B.; Bornhorst, Julia; Schwerdtle, Tanja; Antunes Soares, Felix A.; Aschner, Michael.

In: Neurotoxicity Research, 01.01.2018.

Research output: Contribution to journalArticle

Ruszkiewicz, JA, Teixeira de Macedo, G, Miranda-Vizuete, A, Bowman, AB, Bornhorst, J, Schwerdtle, T, Antunes Soares, FA & Aschner, M 2018, 'Sex-Specific Response of Caenorhabditis elegans to Methylmercury Toxicity', Neurotoxicity Research. https://doi.org/10.1007/s12640-018-9949-4
Ruszkiewicz JA, Teixeira de Macedo G, Miranda-Vizuete A, Bowman AB, Bornhorst J, Schwerdtle T et al. Sex-Specific Response of Caenorhabditis elegans to Methylmercury Toxicity. Neurotoxicity Research. 2018 Jan 1. https://doi.org/10.1007/s12640-018-9949-4
Ruszkiewicz, Joanna A. ; Teixeira de Macedo, Gabriel ; Miranda-Vizuete, Antonio ; Bowman, Aaron B. ; Bornhorst, Julia ; Schwerdtle, Tanja ; Antunes Soares, Felix A. ; Aschner, Michael. / Sex-Specific Response of Caenorhabditis elegans to Methylmercury Toxicity. In: Neurotoxicity Research. 2018.
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