Severe but temporary injury to rabbit orbicularis oculi muscle using dihematoporphyrin ether and laser photochemomyectomy

Jonathan D. Wirtschafter, David P. Slovut, Leif Stordal, Joseph Valentino, Linda Kirschen McLoon

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

The use of local dihematoporphyrin ether (DHE) injections, followed by laser light activation, was investigated as a potential permanent myectomy treatment for muscle spasms, in particular blepharospasm and hemifacial spasm. DHE was injected into the eyelids of rabbits, followed by laser activation, as used in photochemotherapy. Four days after treatment, histological examination indicated that doses of ≥0.5 mg of DHE and laser treatment with an energy density of at least 100 J/cm2 resulted in an almost total destruction of the orbicularis oculi muscle in the treated eyelid. The amount of muscle injury was dependent on both dose of DHE and energy density levels. Histologically, the tarsal glands and conjunctiva were damaged. Glandular tissue was markedly reduced, and the conjuctival epithelium showed hyperplasia and a loss of mucous cells. Six months after DHE and laser treatment, the majority of the muscle tissue had regenerated, although there was evidence of previous injury. While DHE injections combined with laser light activation were lethal to muscle at the site of treatment, this treatment was not permanent. The orbicularis oculi muscle retained its ability to regenerate. However, photochemomyectomy may be studied further as an adjuvant treatment to temporarily injure and debulk large muscles when botulinum toxin is contraindicated due to the large doses involved or as permanent treatment when used togeter with an antimitotic agent such as doxorubicin.

Original languageEnglish (US)
Pages (from-to)171-177
Number of pages7
JournalMovement Disorders
Volume7
Issue number2
DOIs
StatePublished - 1992
Externally publishedYes

Fingerprint

Dihematoporphyrin Ether
Lasers
Rabbits
Muscles
Wounds and Injuries
Eyelids
Therapeutics
Meibomian Glands
Blepharospasm
Hemifacial Spasm
Antimitotic Agents
Light
Injections
Botulinum Toxins
Conjunctiva
Photochemotherapy
Spasm
Doxorubicin
Hyperplasia
Epithelium

Keywords

  • Blepharospasm
  • Facial muscles
  • Photodynamic therapy

ASJC Scopus subject areas

  • Neurology
  • Clinical Neurology

Cite this

Severe but temporary injury to rabbit orbicularis oculi muscle using dihematoporphyrin ether and laser photochemomyectomy. / Wirtschafter, Jonathan D.; Slovut, David P.; Stordal, Leif; Valentino, Joseph; McLoon, Linda Kirschen.

In: Movement Disorders, Vol. 7, No. 2, 1992, p. 171-177.

Research output: Contribution to journalArticle

Wirtschafter, Jonathan D. ; Slovut, David P. ; Stordal, Leif ; Valentino, Joseph ; McLoon, Linda Kirschen. / Severe but temporary injury to rabbit orbicularis oculi muscle using dihematoporphyrin ether and laser photochemomyectomy. In: Movement Disorders. 1992 ; Vol. 7, No. 2. pp. 171-177.
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