Serum stimulation of human fibroblasts

effect on non histones of nuclear ribonucleoprotein and chromatin

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

In quiescent human fibroblasts stimulated to proliferate by fresh medium plus 15% serum, no changes were seen in the incorporation of 3H tryptophan into the protein of nuclear ribonucleoprotein during the first 3 hr following refeeding. This was in contrast to nonhistone chromosomal proteins where the incorporation increased by 90% within 10 min. The density of the formaldehyde fixed nuclear ribonucleoprotein in CsCl was 1.43 to 1.44 g/ml and this also did not change following stimulation. The electrophoretic profile of the proteins of nuclear ribonucleoprotein on SDS gels exhibited a predominant band corresponding to a molecular weight of 44,000 closely trailed by a band at 47,000 and other bands at higher molecular weight. This pattern was not altered by serum stimulation and the same was true for the more complex electrophoretic profile of the chromatin proteins. Following a 10 min pulse of 3H tryptophan at 10 min after stimulation, there was a selective increase in the labeling of nonhistone chromosomal protein of molecular weight 59,000; no change was seen in the labeling of any protein of nuclear ribonucleoprotein.

Original languageEnglish (US)
Pages (from-to)129-136
Number of pages8
JournalJournal of Cellular Physiology
Volume92
Issue number1
StatePublished - 1977
Externally publishedYes

Fingerprint

Ribonucleoproteins
Fibroblasts
Histones
Chromatin
Nuclear Proteins
Non Histone Chromosomal Proteins
Molecular Weight
Molecular weight
Serum
Tryptophan
Labeling
Formaldehyde
Gels
Proteins

ASJC Scopus subject areas

  • Cell Biology
  • Clinical Biochemistry
  • Physiology

Cite this

Serum stimulation of human fibroblasts : effect on non histones of nuclear ribonucleoprotein and chromatin. / Augenlicht, Leonard H.; Lipkin, M.

In: Journal of Cellular Physiology, Vol. 92, No. 1, 1977, p. 129-136.

Research output: Contribution to journalArticle

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