Serum complexes of insulin-like growth factor-1 modulate skeletal integrity and carbohydrate metabolism

Shoshana Yakar, Clifford J. Rosen, Mary L. Bouxsein, Hui (Herb) Sun, Wilson Mejia, Yuki Kawashima, Yingjie Wu, Kelly Emerton, Valerie Williams, Karl Jepsen, Mitchell B. Schaffler, Robert J. Majeska, Oksana Gavrilova, Mariana Gutierrez, David Hwang, Patricia Pennisi, Jan Frystyk, Yves Boisclair, John Pintar, Héctor JasperHoracio Domene, Pinchas Cohen, David Clemmons, Derek Leroith

Research output: Contribution to journalArticle

65 Citations (Scopus)

Abstract

Serum insulin-like growth factor (IGF)-1 is secreted mainly by the liver and circulates bound to IGF-binding proteins (IGFBPs), either as binary complexes or ternary complexes with IGFBP-3 or IGFBP-5 and an acid-labile subunit (ALS). The purpose of this study was to genetically dissect the role of IGF-1 circulatory complexes in somatic growth, skeletal integrity, and metabolism. Phenotypic comparisons of controls and four mouse lines with genetic IGF-1 deficits-liver-specific IGF-1 deficiency (LID), ALS knockout (ALSKO), IGFBP-3 (BP3) knockout, and a triply deficient LID/ALSKO/BP3 line-produced several novel findings. 1) All deficient strains had decreased serum IGF-1 levels, but this neither predicted growth potential or skeletal integrity nor defined growth hormone secretion or metabolic abnormalities. 2) IGF-1 deficiency affected development of both cortical and trabecular bone differently, effects apparently dependent on the presence of different circulating IGF-1 complexes. 3) IGFBP-3 deficiency resulted in increased linear growth. In summary, each IGF-1 complex constituent appears to play a distinct role in determining skeletal phenotype, with different effects on cortical and trabecular bone compartments. -Yakar, S., Rosen, C. J., Bouxsein, M. L., Sun, H., Mejia, W., Kawashima, Y., Wu, Y., Emerton, K., Williams, V., Jepsen, K., Schaffler, M. B., Majeska, R. J., Gavrilova, O., Gutierrez, M., Hwang, D., Pennisi, P., Frystyk, J., Boisclair, Y., Pintar, J., Jasper, H., Domene, H., Cohen, P., Clemmons, D., LeRoith, D. Serum complexes of insulin-like growth factor-1 modulate skeletal integrity and carbohydrate metabolism. FASEB J. 23, 709-719 (2009)

Original languageEnglish (US)
Pages (from-to)709-719
Number of pages11
JournalFASEB Journal
Volume23
Issue number3
DOIs
StatePublished - Mar 2009
Externally publishedYes

Fingerprint

Carbohydrate Metabolism
Somatomedins
Serum
Insulin-Like Growth Factor Binding Protein 3
Liver
Bone
Growth
Insulin-Like Growth Factor Binding Protein 5
Protein Deficiency
Insulin-Like Growth Factor Binding Proteins
Acids
Solar System
Insulin-Like Growth Factor I
Metabolism
Sun
Growth Hormone
Phenotype

Keywords

  • Binary complex
  • Cortical bone
  • Osteoblast
  • Osteoclast
  • Ternary complex
  • Trabecular bone

ASJC Scopus subject areas

  • Biochemistry
  • Biotechnology
  • Genetics
  • Molecular Biology

Cite this

Yakar, S., Rosen, C. J., Bouxsein, M. L., Sun, H. H., Mejia, W., Kawashima, Y., ... Leroith, D. (2009). Serum complexes of insulin-like growth factor-1 modulate skeletal integrity and carbohydrate metabolism. FASEB Journal, 23(3), 709-719. https://doi.org/10.1096/fj.08-118976

Serum complexes of insulin-like growth factor-1 modulate skeletal integrity and carbohydrate metabolism. / Yakar, Shoshana; Rosen, Clifford J.; Bouxsein, Mary L.; Sun, Hui (Herb); Mejia, Wilson; Kawashima, Yuki; Wu, Yingjie; Emerton, Kelly; Williams, Valerie; Jepsen, Karl; Schaffler, Mitchell B.; Majeska, Robert J.; Gavrilova, Oksana; Gutierrez, Mariana; Hwang, David; Pennisi, Patricia; Frystyk, Jan; Boisclair, Yves; Pintar, John; Jasper, Héctor; Domene, Horacio; Cohen, Pinchas; Clemmons, David; Leroith, Derek.

In: FASEB Journal, Vol. 23, No. 3, 03.2009, p. 709-719.

Research output: Contribution to journalArticle

Yakar, S, Rosen, CJ, Bouxsein, ML, Sun, HH, Mejia, W, Kawashima, Y, Wu, Y, Emerton, K, Williams, V, Jepsen, K, Schaffler, MB, Majeska, RJ, Gavrilova, O, Gutierrez, M, Hwang, D, Pennisi, P, Frystyk, J, Boisclair, Y, Pintar, J, Jasper, H, Domene, H, Cohen, P, Clemmons, D & Leroith, D 2009, 'Serum complexes of insulin-like growth factor-1 modulate skeletal integrity and carbohydrate metabolism', FASEB Journal, vol. 23, no. 3, pp. 709-719. https://doi.org/10.1096/fj.08-118976
Yakar, Shoshana ; Rosen, Clifford J. ; Bouxsein, Mary L. ; Sun, Hui (Herb) ; Mejia, Wilson ; Kawashima, Yuki ; Wu, Yingjie ; Emerton, Kelly ; Williams, Valerie ; Jepsen, Karl ; Schaffler, Mitchell B. ; Majeska, Robert J. ; Gavrilova, Oksana ; Gutierrez, Mariana ; Hwang, David ; Pennisi, Patricia ; Frystyk, Jan ; Boisclair, Yves ; Pintar, John ; Jasper, Héctor ; Domene, Horacio ; Cohen, Pinchas ; Clemmons, David ; Leroith, Derek. / Serum complexes of insulin-like growth factor-1 modulate skeletal integrity and carbohydrate metabolism. In: FASEB Journal. 2009 ; Vol. 23, No. 3. pp. 709-719.
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AU - Mejia, Wilson

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AU - Wu, Yingjie

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AU - Boisclair, Yves

AU - Pintar, John

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