Serum autoantibodies in pristane induced lupus are regulated by neutrophil gelatinase associated lipocalin

Rahul D. Pawar, Beatrice Goilav, Yumin Xia, Haoyang Zhuang, Leal Herlitz, Westley H. Reeves, Chaim Putterman

Research output: Contribution to journalArticle

11 Scopus citations

Abstract

The onset of autoantibodies in systemic autoimmunity can be the result of a breakdown in tolerance at multiple checkpoints. Genetic, hormonal, and immunological factors can combine with environmental influences to accelerate the onset of disease and aggravate disease outcome. Here, we describe a novel mechanism relating to the regulatory role of Neutrophil Gelatinase Associated Lipocalin (NGAL) in modulating the levels of autoantibodies in pristane induced lupus. Following a single injection of pristane intraperitoneally, NGAL expression was induced in both the serum and spleen. Furthermore, NGAL deficient mice were more susceptible to the induction of pristane stimulated autoimmunity, and displayed higher numbers of autoantibody secreting cells and increased expression of activation induced cytidine deaminase (AID) and other inflammatory mediators in the spleen. In contrast, kidney damage was milder in NGAL deficient mice, indicating that NGAL was detrimental in autoantibody mediated kidney disease. These studies indicate that NGAL plays differential roles in different tissues in the context of lupus, and suggest a previously unrecognized role for NGAL in adaptive immunity.

Original languageEnglish (US)
Pages (from-to)49-65
Number of pages17
JournalClinical Immunology
Volume154
Issue number1
DOIs
StatePublished - Sep 2014

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Keywords

  • Autoantibodies
  • Lipocalin-2
  • NGAL
  • Pristane
  • SLE

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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