Serological association between spirorchidiasis, herpesvirus infection, and fibropapillomatosis in green turtles from Florida

Lawrence H. Herbst, Ellis C. Greiner, Llewellyn M. Ehrhart, Dean A. Bagley, Paul A. Klein

Research output: Contribution to journalArticle

35 Citations (Scopus)

Abstract

Serodiagnostic tests for detecting green turtle (Chelonia mydas) antibody responses were developed to test the strength of association between exposure to spirorchid trematode antigens or herpesvirus antigens and having green turtle fibropapillomatosis (GTFP). Plasma samples from 46 captive-reared green turtles, including paired pre- and 1-yr post-inoculation samples from 12 turtles with experimentally induced GTFP, were found by enzyme-linked immunosorbent assay (ELISA) to be negative for antibodies to adult spirorchid (Learedius learedi) antigens. In contrast, all 12 turtles that developed experimentally induced GTFP converted within 1 yr from having negative to positive antibody reactivity to GTFP-associated herpesvirus antigens, whereas the three controls and four turtles that failed to develop tumors remained negative. Plasma samples from 104 free-ranging green turtles from two Florida (USA) coastal feeding grounds with different GTFP prevalences were tested by ELISA for antibodies to L. learedi adult antigens; and there was no statistically significant association between antibody prevalence and sampling site. When a low optical density cutoff value (0.15) was used to interpret ELISA results, 98% of the turtles from each site were spirorchid antibody-positive and there was no association between antibody reactivity to spirorchids and GTFP status. When a higher negative cutoff value was used, however, a statistically significant association between antibody reactivity to spirorchids and GTFP-free status was found. These results suggest that spirorchids do not have a role in GTFP pathogenesis. All 20 of the tumor-bearing lagoon turtles had antibodies to herpesvirus antigens whereas only two (10%) of the tumor-free reef turtles had detectable anti-herpesvirus reactivity. The strong association between antibody reactivity to herpesvirus antigens and GTFP status in both captive-reared and free-ranging turtles is consistent with the hypothesis that the transmissible agent that causes GTFP is a herpesvirus.

Original languageEnglish (US)
Pages (from-to)496-507
Number of pages12
JournalJournal of Wildlife Diseases
Volume34
Issue number3
StatePublished - Jul 1998
Externally publishedYes

Fingerprint

Herpesviridae Infections
Chelonia mydas
Turtles
turtle
turtles
antibody
antibodies
antigen
Herpesviridae
antigens
Antibodies
Antigens
enzyme-linked immunosorbent assay
Herpesviridae infections
infection
tumor
neoplasms
Enzyme-Linked Immunosorbent Assay
assay
enzyme

Keywords

  • Chelonia mydas
  • Fibropapillomatosis
  • Green turtle
  • Herpesvirus
  • Plasma antibodies
  • Spirorchidiasis

ASJC Scopus subject areas

  • veterinary(all)

Cite this

Serological association between spirorchidiasis, herpesvirus infection, and fibropapillomatosis in green turtles from Florida. / Herbst, Lawrence H.; Greiner, Ellis C.; Ehrhart, Llewellyn M.; Bagley, Dean A.; Klein, Paul A.

In: Journal of Wildlife Diseases, Vol. 34, No. 3, 07.1998, p. 496-507.

Research output: Contribution to journalArticle

Herbst, Lawrence H. ; Greiner, Ellis C. ; Ehrhart, Llewellyn M. ; Bagley, Dean A. ; Klein, Paul A. / Serological association between spirorchidiasis, herpesvirus infection, and fibropapillomatosis in green turtles from Florida. In: Journal of Wildlife Diseases. 1998 ; Vol. 34, No. 3. pp. 496-507.
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abstract = "Serodiagnostic tests for detecting green turtle (Chelonia mydas) antibody responses were developed to test the strength of association between exposure to spirorchid trematode antigens or herpesvirus antigens and having green turtle fibropapillomatosis (GTFP). Plasma samples from 46 captive-reared green turtles, including paired pre- and 1-yr post-inoculation samples from 12 turtles with experimentally induced GTFP, were found by enzyme-linked immunosorbent assay (ELISA) to be negative for antibodies to adult spirorchid (Learedius learedi) antigens. In contrast, all 12 turtles that developed experimentally induced GTFP converted within 1 yr from having negative to positive antibody reactivity to GTFP-associated herpesvirus antigens, whereas the three controls and four turtles that failed to develop tumors remained negative. Plasma samples from 104 free-ranging green turtles from two Florida (USA) coastal feeding grounds with different GTFP prevalences were tested by ELISA for antibodies to L. learedi adult antigens; and there was no statistically significant association between antibody prevalence and sampling site. When a low optical density cutoff value (0.15) was used to interpret ELISA results, 98{\%} of the turtles from each site were spirorchid antibody-positive and there was no association between antibody reactivity to spirorchids and GTFP status. When a higher negative cutoff value was used, however, a statistically significant association between antibody reactivity to spirorchids and GTFP-free status was found. These results suggest that spirorchids do not have a role in GTFP pathogenesis. All 20 of the tumor-bearing lagoon turtles had antibodies to herpesvirus antigens whereas only two (10{\%}) of the tumor-free reef turtles had detectable anti-herpesvirus reactivity. The strong association between antibody reactivity to herpesvirus antigens and GTFP status in both captive-reared and free-ranging turtles is consistent with the hypothesis that the transmissible agent that causes GTFP is a herpesvirus.",
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