Sequences in the human immunodeficiency virus type 1 U3 region required for in vivo and in vitro integration

A. S. Reicin, G. Kalpana, S. Paik, S. Marmon, S. Goff

Research output: Contribution to journalArticle

22 Scopus citations


A series of mutants with alterations in the U3 region of the human immunodeficiency virus type 1 long terminal repeat were made, and the effects of these mutations were evaluated both in vitro and in vivo. When the subterminal 6 to 8 nucleotides of the U3 long terminal repeat were mutated, the resulting provirus was unable to efficiently replicate in vivo, and a mutant oligonucleotide which mimicked the mutation could not be efficiently cleaved but could be joined to target DNA by wild-type recombinant integrase protein in vitro. These results suggest that this region is important in the specific recognition of the vital DNA by the integrase protein.

Original languageEnglish (US)
Pages (from-to)5904-5907
Number of pages4
JournalJournal of virology
Issue number9
StatePublished - Jan 1 1995


ASJC Scopus subject areas

  • Microbiology
  • Immunology
  • Insect Science
  • Virology

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