Abstract
A series of mutants with alterations in the U3 region of the human immunodeficiency virus type 1 long terminal repeat were made, and the effects of these mutations were evaluated both in vitro and in vivo. When the subterminal 6 to 8 nucleotides of the U3 long terminal repeat were mutated, the resulting provirus was unable to efficiently replicate in vivo, and a mutant oligonucleotide which mimicked the mutation could not be efficiently cleaved but could be joined to target DNA by wild-type recombinant integrase protein in vitro. These results suggest that this region is important in the specific recognition of the vital DNA by the integrase protein.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 5904-5907 |
| Number of pages | 4 |
| Journal | Journal of Virology |
| Volume | 69 |
| Issue number | 9 |
| State | Published - 1995 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Immunology
Cite this
Sequences in the human immunodeficiency virus type 1 U3 region required for in vivo and in vitro integration. / Reicin, A. S.; Kalpana, Ganjam V.; Paik, S.; Marmon, S.; Goff, S.
In: Journal of Virology, Vol. 69, No. 9, 1995, p. 5904-5907.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Sequences in the human immunodeficiency virus type 1 U3 region required for in vivo and in vitro integration
AU - Reicin, A. S.
AU - Kalpana, Ganjam V.
AU - Paik, S.
AU - Marmon, S.
AU - Goff, S.
PY - 1995
Y1 - 1995
N2 - A series of mutants with alterations in the U3 region of the human immunodeficiency virus type 1 long terminal repeat were made, and the effects of these mutations were evaluated both in vitro and in vivo. When the subterminal 6 to 8 nucleotides of the U3 long terminal repeat were mutated, the resulting provirus was unable to efficiently replicate in vivo, and a mutant oligonucleotide which mimicked the mutation could not be efficiently cleaved but could be joined to target DNA by wild-type recombinant integrase protein in vitro. These results suggest that this region is important in the specific recognition of the vital DNA by the integrase protein.
AB - A series of mutants with alterations in the U3 region of the human immunodeficiency virus type 1 long terminal repeat were made, and the effects of these mutations were evaluated both in vitro and in vivo. When the subterminal 6 to 8 nucleotides of the U3 long terminal repeat were mutated, the resulting provirus was unable to efficiently replicate in vivo, and a mutant oligonucleotide which mimicked the mutation could not be efficiently cleaved but could be joined to target DNA by wild-type recombinant integrase protein in vitro. These results suggest that this region is important in the specific recognition of the vital DNA by the integrase protein.
UR - http://www.scopus.com/inward/record.url?scp=0029122532&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0029122532&partnerID=8YFLogxK
M3 - Article
VL - 69
SP - 5904
EP - 5907
JO - Journal of Virology
JF - Journal of Virology
SN - 0022-538X
IS - 9
ER -