Sequences in the human immunodeficiency virus type 1 U3 region required for in vivo and in vitro integration

Alise S. Reicin, Ganjam Kalpana, Sandra Paik, Shana Marmon, Stephen Goff

Research output: Contribution to journalArticle

22 Scopus citations

Abstract

A series of mutants with alterations in the U3 region of the human immunodeficiency virus type 1 long terminal repeat were made, and the effects of these mutations were evaluated both in vitro and in vivo. When the subterminal 6 to 8 nucleotides of the U3 long terminal repeat were mutated, the resulting provirus was unable to efficiently replicate in vivo, and a mutant oligonucleotide which mimicked the mutation could not be efficiently cleaved but could be joined to target DNA by wild-type recombinant integrase protein in vitro. These results suggest that this region is important in the specific recognition of the vital DNA by the integrase protein.

Original languageEnglish (US)
Pages (from-to)5904-5907
Number of pages4
JournalJournal of virology
Volume69
Issue number9
DOIs
StatePublished - Sep 1995

ASJC Scopus subject areas

  • Microbiology
  • Immunology
  • Insect Science
  • Virology

Fingerprint Dive into the research topics of 'Sequences in the human immunodeficiency virus type 1 U3 region required for in vivo and in vitro integration'. Together they form a unique fingerprint.

  • Cite this