Sequence similarity between the 73-kilodalton protein of mammalian CPSF and a subunit of yeast polyadenylation factor I

Andreas Jenny, Lionel Minvielle-Sebastia, Pascal J. Preker, Walter Keller

Research output: Contribution to journalArticle

92 Scopus citations

Abstract

The 3' ends of most eukaryotic messenger RNAs are generated by endonucleolytic cleavage and polyadenylation. In mammals, the cleavage and polyadenylation specificity factor (CPSF) plays a central role in both steps of the processing reaction. Here, the cloning of the 73-kilodalton subunit of CPSF is reported. Sequence analyses revealed that a yeast protein (Ysh1) was highly similar to the 73-kD polypeptide. Ysh1 constitutes a new subunit of polyadenylation factor I (PFI), which has a role in yeast pre-mRNA 3'-end formation. This finding was unexpected because in contrast to CPSF, PFI is only required for the polyadenylation reaction. These results contribute to the understanding of how 3'-end processing factors may have evolved.

Original languageEnglish (US)
Pages (from-to)1514-1517
Number of pages4
JournalScience
Volume274
Issue number5292
DOIs
StatePublished - Nov 29 1996
Externally publishedYes

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