Septate junction disruption and surface reorganization by non-lethal Ca2+ shock

William Reed, Peter Satir

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Using potassium pyroantimonate as a Ca2+ chelating agent, we have been able to open the belt desmosome that mechanically couples adjacent ciliated lateral (L) gill epithelial cells of freshwater mussels. Subsequent transfer of the mechanically uncoupled epithelia to solutions containing millimolar Ca2+ results in dramatic alterations in cell shape and partial disruption and apical reorientation of L cell septate junction. The cells remain viable throughout the procedure as determined by the persistence of ciliary activity, although disruption is accompanied by a separation of neighboring groups of cilia and a switch from metachronal to synchronous L cell ciliary beat co-ordination.

Original languageEnglish (US)
Pages (from-to)469-478
Number of pages10
JournalCell Biology International Reports
Volume5
Issue number5
DOIs
StatePublished - 1981

Fingerprint

Shock
Desmosomes
Intercellular Junctions
Cell Shape
Cilia
Bivalvia
Chelating Agents
Fresh Water
Epithelium
Epithelial Cells
potassium antimonate

ASJC Scopus subject areas

  • Cell Biology

Cite this

Septate junction disruption and surface reorganization by non-lethal Ca2+ shock. / Reed, William; Satir, Peter.

In: Cell Biology International Reports, Vol. 5, No. 5, 1981, p. 469-478.

Research output: Contribution to journalArticle

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