Abstract
Using potassium pyroantimonate as a Ca2+ chelating agent, we have been able to open the belt desmosome that mechanically couples adjacent ciliated lateral (L) gill epithelial cells of freshwater mussels. Subsequent transfer of the mechanically uncoupled epithelia to solutions containing millimolar Ca2+ results in dramatic alterations in cell shape and partial disruption and apical reorientation of L cell septate junction. The cells remain viable throughout the procedure as determined by the persistence of ciliary activity, although disruption is accompanied by a separation of neighboring groups of cilia and a switch from metachronal to synchronous L cell ciliary beat co-ordination.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 469-478 |
| Number of pages | 10 |
| Journal | Cell Biology International Reports |
| Volume | 5 |
| Issue number | 5 |
| DOIs | |
| State | Published - 1981 |
Fingerprint
ASJC Scopus subject areas
- Cell Biology
Cite this
Septate junction disruption and surface reorganization by non-lethal Ca2+ shock. / Reed, William; Satir, Peter.
In: Cell Biology International Reports, Vol. 5, No. 5, 1981, p. 469-478.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Septate junction disruption and surface reorganization by non-lethal Ca2+ shock
AU - Reed, William
AU - Satir, Peter
PY - 1981
Y1 - 1981
N2 - Using potassium pyroantimonate as a Ca2+ chelating agent, we have been able to open the belt desmosome that mechanically couples adjacent ciliated lateral (L) gill epithelial cells of freshwater mussels. Subsequent transfer of the mechanically uncoupled epithelia to solutions containing millimolar Ca2+ results in dramatic alterations in cell shape and partial disruption and apical reorientation of L cell septate junction. The cells remain viable throughout the procedure as determined by the persistence of ciliary activity, although disruption is accompanied by a separation of neighboring groups of cilia and a switch from metachronal to synchronous L cell ciliary beat co-ordination.
AB - Using potassium pyroantimonate as a Ca2+ chelating agent, we have been able to open the belt desmosome that mechanically couples adjacent ciliated lateral (L) gill epithelial cells of freshwater mussels. Subsequent transfer of the mechanically uncoupled epithelia to solutions containing millimolar Ca2+ results in dramatic alterations in cell shape and partial disruption and apical reorientation of L cell septate junction. The cells remain viable throughout the procedure as determined by the persistence of ciliary activity, although disruption is accompanied by a separation of neighboring groups of cilia and a switch from metachronal to synchronous L cell ciliary beat co-ordination.
UR - http://www.scopus.com/inward/record.url?scp=0019414125&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0019414125&partnerID=8YFLogxK
U2 - 10.1016/0309-1651(81)90174-0
DO - 10.1016/0309-1651(81)90174-0
M3 - Article
C2 - 7195317
AN - SCOPUS:0019414125
VL - 5
SP - 469
EP - 478
JO - Cell Biology International
JF - Cell Biology International
SN - 1065-6995
IS - 5
ER -