TY - JOUR
T1 - Separation of Carrier-Free Holmium-166 from Neutron-Irradiated Dysprosium Targets
AU - Dadachova, Ekaterina
AU - Mirzadeh, Saed
AU - Lambrecht, Richard M.
AU - Hetherington, Eric L.
AU - Knapp, F. F.
PY - 1994/12/1
Y1 - 1994/12/1
N2 - Holmium-166 (166Ho, t1/2 = 26.4 h) is utilized in radiotherapeutic applications such as radioimmunospecific pharmaceuticals, bone marrow ablation, and radiation synovectomy. High specific activity 166Ho can be obtained from the decay of dysprosium-166 (166Dy, t1/2 = 81.5 h). Dysprosium-166 is produced by the 164Dy[n,γ]165Dy-[n,γ]166Dy reaction in a nuclear reactor. The applicability of reversed phase ion-exchange chromatographic methods was demonstrated for the separation of carrier-free 166Ho from milligram quantities of 164Dy2O3 irradiated targets. An efficient and quantitative separation was achieved utilizing a metal-free HPLC system with Dowex AG 50WX12 or Aminex-A5 cation exchangers and α-hydroxyisobutyric acid (α-HIBA) as the eluent (0.085 M, pH = 4.3 adjusted with NH4OH). The Aminex-A5 column gave a separation factor of #x223C;103 between Ho and Dy. Subsequent to the acidic destruction of the Ho-HIBA complex, Ho3+ was further purified on a small cation-exchange column from acidic chloride solutions. The separation was achieved within 2 h, with a 95% overall radiochemical yield for carrier-free 166Ho with a Dy breakthrough of #x003C;0.1%.
AB - Holmium-166 (166Ho, t1/2 = 26.4 h) is utilized in radiotherapeutic applications such as radioimmunospecific pharmaceuticals, bone marrow ablation, and radiation synovectomy. High specific activity 166Ho can be obtained from the decay of dysprosium-166 (166Dy, t1/2 = 81.5 h). Dysprosium-166 is produced by the 164Dy[n,γ]165Dy-[n,γ]166Dy reaction in a nuclear reactor. The applicability of reversed phase ion-exchange chromatographic methods was demonstrated for the separation of carrier-free 166Ho from milligram quantities of 164Dy2O3 irradiated targets. An efficient and quantitative separation was achieved utilizing a metal-free HPLC system with Dowex AG 50WX12 or Aminex-A5 cation exchangers and α-hydroxyisobutyric acid (α-HIBA) as the eluent (0.085 M, pH = 4.3 adjusted with NH4OH). The Aminex-A5 column gave a separation factor of #x223C;103 between Ho and Dy. Subsequent to the acidic destruction of the Ho-HIBA complex, Ho3+ was further purified on a small cation-exchange column from acidic chloride solutions. The separation was achieved within 2 h, with a 95% overall radiochemical yield for carrier-free 166Ho with a Dy breakthrough of #x003C;0.1%.
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U2 - 10.1021/ac00095a024
DO - 10.1021/ac00095a024
M3 - Article
AN - SCOPUS:0000071567
SN - 0003-2700
VL - 66
SP - 4272
EP - 4277
JO - Analytical Chemistry
JF - Analytical Chemistry
IS - 23
ER -