Separation and quantification by gas chromatography-mass spectrometry of arabinitol enantiomers to aid the differential diagnosis of disseminated candidiasis

John Roboz, Edward Nieves, J. F. Holland

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

To differentiate increased arabinitol due to fungal (only d-arabinitol) and nonfungal origin, O-trifluoroacetyl derivatives of the enantiomers were separated using α-perpentylated cyclodextrin columns and measured by selected ion monitoring. Mean±S.D. d/l in normal serum: 1.40±0.42. l/l ratios > 2.24, defined as normal mean+2S.D., were considered outside normal range. D/L was > 2.2 in 10 of 12 confirmed candidiasis cases with one false negative and one borderline. Renal dysfunction without candidiasis yielded normal d/l despite high arabinitol concentrations. d/l in normal urine was nearly identical to that in serum despite 60 times larger concentration. d/l ratios, determined by peak heights or areas, could be used without the need to determine concentrations.

Original languageEnglish (US)
Pages (from-to)413-426
Number of pages14
JournalJournal of Chromatography A
Volume500
Issue numberC
DOIs
StatePublished - Feb 2 1990
Externally publishedYes

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Enantiomers
Candidiasis
Cyclodextrins
Gas chromatography
Gas Chromatography-Mass Spectrometry
Mass spectrometry
Differential Diagnosis
Ions
Derivatives
Monitoring
Serum
Reference Values
Urine
Kidney

ASJC Scopus subject areas

  • Analytical Chemistry
  • Clinical Biochemistry
  • Molecular Medicine

Cite this

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abstract = "To differentiate increased arabinitol due to fungal (only d-arabinitol) and nonfungal origin, O-trifluoroacetyl derivatives of the enantiomers were separated using α-perpentylated cyclodextrin columns and measured by selected ion monitoring. Mean±S.D. d/l in normal serum: 1.40±0.42. l/l ratios > 2.24, defined as normal mean+2S.D., were considered outside normal range. D/L was > 2.2 in 10 of 12 confirmed candidiasis cases with one false negative and one borderline. Renal dysfunction without candidiasis yielded normal d/l despite high arabinitol concentrations. d/l in normal urine was nearly identical to that in serum despite 60 times larger concentration. d/l ratios, determined by peak heights or areas, could be used without the need to determine concentrations.",
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T1 - Separation and quantification by gas chromatography-mass spectrometry of arabinitol enantiomers to aid the differential diagnosis of disseminated candidiasis

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AU - Nieves, Edward

AU - Holland, J. F.

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AB - To differentiate increased arabinitol due to fungal (only d-arabinitol) and nonfungal origin, O-trifluoroacetyl derivatives of the enantiomers were separated using α-perpentylated cyclodextrin columns and measured by selected ion monitoring. Mean±S.D. d/l in normal serum: 1.40±0.42. l/l ratios > 2.24, defined as normal mean+2S.D., were considered outside normal range. D/L was > 2.2 in 10 of 12 confirmed candidiasis cases with one false negative and one borderline. Renal dysfunction without candidiasis yielded normal d/l despite high arabinitol concentrations. d/l in normal urine was nearly identical to that in serum despite 60 times larger concentration. d/l ratios, determined by peak heights or areas, could be used without the need to determine concentrations.

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