Sensitivity of the periodontal pathogen Aggregatibacter actinomycetemcomitans at mildly acidic pH

Mrinal K. Bhattacharjee, Claiborne B. Childs, Emdad Ali

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Background: Aggregatibacter actinomycetemcomitans (previously Actinobacillus actinomycetemcomitans), a capnophilic facultative anaerobe, is associated with localized aggressive periodontitis and endocarditis. When grown in broth, the cells begin to die rapidly after overnight growth. The cells also often lose viability on plates within a few days. The aim of this study is to identify the cause of the rapid loss of cell viability. Methods: Cell viabilities, as measured by colony forming units, were determined for cells obtained from isolated colonies and from the dense part of a streak on plates. The effect of pH on cell viability was determined by growing cells in broth at various initial glucose concentrations and with or without added bicarbonate. Results: A. actinomycetemcomitans cells were highly sensitive to even a mildly acidic pH of ≈6. Because the bacteria grew at a glucose concentration that is commonly used in many laboratories, there was a dramatic decrease in cell viability as the pH went

Original languageEnglish (US)
Pages (from-to)917-925
Number of pages9
JournalJournal of Periodontology
Volume82
Issue number6
DOIs
StatePublished - Jun 2011
Externally publishedYes

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Aggregatibacter actinomycetemcomitans
Cell Survival
Aggressive Periodontitis
Glucose
Bicarbonates
Endocarditis
Stem Cells
Bacteria
Growth

Keywords

  • Aggressive periodontitis
  • Antimicrobials
  • Gingival crevicular fluid
  • Microbiology
  • Periodontitis

ASJC Scopus subject areas

  • Periodontics

Cite this

Sensitivity of the periodontal pathogen Aggregatibacter actinomycetemcomitans at mildly acidic pH. / Bhattacharjee, Mrinal K.; Childs, Claiborne B.; Ali, Emdad.

In: Journal of Periodontology, Vol. 82, No. 6, 06.2011, p. 917-925.

Research output: Contribution to journalArticle

Bhattacharjee, Mrinal K. ; Childs, Claiborne B. ; Ali, Emdad. / Sensitivity of the periodontal pathogen Aggregatibacter actinomycetemcomitans at mildly acidic pH. In: Journal of Periodontology. 2011 ; Vol. 82, No. 6. pp. 917-925.
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