TY - JOUR
T1 - Sensitive LC MS quantitative analysis of carbohydrates by Cs+ attachment
AU - Rogatsky, Eduard
AU - Jayatillake, Harsha
AU - Goswami, Gayotri
AU - Tomuta, Vlad
AU - Stein, Daniel
N1 - Funding Information:
The authors thank Greg Cruikshank and Louis Vele for excellent technical assistance, Phenomenex for excellent customer and technical support, and Professor Irving Listowsky for helpful discussions and comments on the manuscript. This study was supported by grants from the American Diabetes Association, NIH/NCRR and NIDDK: MO1-RR12248, R01 DK61644-01.
PY - 2005/11
Y1 - 2005/11
N2 - The development of a sensitive assay for the quantitative analysis of carbohydrates from human plasma using LC/MS/MS is described in this paper. After sample preparation, carbohydrates were cationized by Cs+ after their separation by normal phase liquid chromatography on an amino based column. Cesium is capable of forming a quasi-molecular ion [M + Cs]+ with neutral carbohydrate molecules in the positive ion mode of electrospray ionization mass spectrometry. The mass spectrometer was operated in multiple reaction monitoring mode, and transitions [M + 133] → 133 were monitored (M, carbohydrate molecular weight). The new method is robust, highly sensitive, rapid, and does not require postcolumn addition or derivatization. It is useful in clinical research for measurement of carbohydrate molecules by isotope dilution assay.
AB - The development of a sensitive assay for the quantitative analysis of carbohydrates from human plasma using LC/MS/MS is described in this paper. After sample preparation, carbohydrates were cationized by Cs+ after their separation by normal phase liquid chromatography on an amino based column. Cesium is capable of forming a quasi-molecular ion [M + Cs]+ with neutral carbohydrate molecules in the positive ion mode of electrospray ionization mass spectrometry. The mass spectrometer was operated in multiple reaction monitoring mode, and transitions [M + 133] → 133 were monitored (M, carbohydrate molecular weight). The new method is robust, highly sensitive, rapid, and does not require postcolumn addition or derivatization. It is useful in clinical research for measurement of carbohydrate molecules by isotope dilution assay.
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U2 - 10.1016/j.jasms.2005.07.017
DO - 10.1016/j.jasms.2005.07.017
M3 - Article
C2 - 16182559
AN - SCOPUS:26944455186
SN - 1044-0305
VL - 16
SP - 1805
EP - 1811
JO - Journal of the American Society for Mass Spectrometry
JF - Journal of the American Society for Mass Spectrometry
IS - 11
ER -