Sensitive LC MS quantitative analysis of carbohydrates by Cs+ attachment

Eduard Rogatsky, Harsha Jayatillake, Gayotri Goswami, Vlad A. Tomuta, Daniel T. Stein

Research output: Contribution to journalArticle

45 Citations (Scopus)

Abstract

The development of a sensitive assay for the quantitative analysis of carbohydrates from human plasma using LC/MS/MS is described in this paper. After sample preparation, carbohydrates were cationized by Cs+ after their separation by normal phase liquid chromatography on an amino based column. Cesium is capable of forming a quasi-molecular ion [M + Cs]+ with neutral carbohydrate molecules in the positive ion mode of electrospray ionization mass spectrometry. The mass spectrometer was operated in multiple reaction monitoring mode, and transitions [M + 133] → 133 were monitored (M, carbohydrate molecular weight). The new method is robust, highly sensitive, rapid, and does not require postcolumn addition or derivatization. It is useful in clinical research for measurement of carbohydrate molecules by isotope dilution assay.

Original languageEnglish (US)
Pages (from-to)1805-1811
Number of pages7
JournalJournal of the American Society for Mass Spectrometry
Volume16
Issue number11
DOIs
StatePublished - Nov 2005

Fingerprint

Carbohydrates
Chemical analysis
Assays
Ions
Plasma (human)
Electrospray ionization
Cesium
Molecules
Electrospray Ionization Mass Spectrometry
Liquid chromatography
Mass spectrometers
Liquid Chromatography
Isotopes
Dilution
Mass spectrometry
Molecular Weight
Positive ions
Molecular weight
Monitoring
Research

ASJC Scopus subject areas

  • Structural Biology
  • Spectroscopy

Cite this

Sensitive LC MS quantitative analysis of carbohydrates by Cs+ attachment. / Rogatsky, Eduard; Jayatillake, Harsha; Goswami, Gayotri; Tomuta, Vlad A.; Stein, Daniel T.

In: Journal of the American Society for Mass Spectrometry, Vol. 16, No. 11, 11.2005, p. 1805-1811.

Research output: Contribution to journalArticle

@article{48918400ab2241ac937a0a7bbeee6bfb,
title = "Sensitive LC MS quantitative analysis of carbohydrates by Cs+ attachment",
abstract = "The development of a sensitive assay for the quantitative analysis of carbohydrates from human plasma using LC/MS/MS is described in this paper. After sample preparation, carbohydrates were cationized by Cs+ after their separation by normal phase liquid chromatography on an amino based column. Cesium is capable of forming a quasi-molecular ion [M + Cs]+ with neutral carbohydrate molecules in the positive ion mode of electrospray ionization mass spectrometry. The mass spectrometer was operated in multiple reaction monitoring mode, and transitions [M + 133] → 133 were monitored (M, carbohydrate molecular weight). The new method is robust, highly sensitive, rapid, and does not require postcolumn addition or derivatization. It is useful in clinical research for measurement of carbohydrate molecules by isotope dilution assay.",
author = "Eduard Rogatsky and Harsha Jayatillake and Gayotri Goswami and Tomuta, {Vlad A.} and Stein, {Daniel T.}",
year = "2005",
month = "11",
doi = "10.1016/j.jasms.2005.07.017",
language = "English (US)",
volume = "16",
pages = "1805--1811",
journal = "Journal of the American Society for Mass Spectrometry",
issn = "1044-0305",
publisher = "Springer New York",
number = "11",

}

TY - JOUR

T1 - Sensitive LC MS quantitative analysis of carbohydrates by Cs+ attachment

AU - Rogatsky, Eduard

AU - Jayatillake, Harsha

AU - Goswami, Gayotri

AU - Tomuta, Vlad A.

AU - Stein, Daniel T.

PY - 2005/11

Y1 - 2005/11

N2 - The development of a sensitive assay for the quantitative analysis of carbohydrates from human plasma using LC/MS/MS is described in this paper. After sample preparation, carbohydrates were cationized by Cs+ after their separation by normal phase liquid chromatography on an amino based column. Cesium is capable of forming a quasi-molecular ion [M + Cs]+ with neutral carbohydrate molecules in the positive ion mode of electrospray ionization mass spectrometry. The mass spectrometer was operated in multiple reaction monitoring mode, and transitions [M + 133] → 133 were monitored (M, carbohydrate molecular weight). The new method is robust, highly sensitive, rapid, and does not require postcolumn addition or derivatization. It is useful in clinical research for measurement of carbohydrate molecules by isotope dilution assay.

AB - The development of a sensitive assay for the quantitative analysis of carbohydrates from human plasma using LC/MS/MS is described in this paper. After sample preparation, carbohydrates were cationized by Cs+ after their separation by normal phase liquid chromatography on an amino based column. Cesium is capable of forming a quasi-molecular ion [M + Cs]+ with neutral carbohydrate molecules in the positive ion mode of electrospray ionization mass spectrometry. The mass spectrometer was operated in multiple reaction monitoring mode, and transitions [M + 133] → 133 were monitored (M, carbohydrate molecular weight). The new method is robust, highly sensitive, rapid, and does not require postcolumn addition or derivatization. It is useful in clinical research for measurement of carbohydrate molecules by isotope dilution assay.

UR - http://www.scopus.com/inward/record.url?scp=26944455186&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=26944455186&partnerID=8YFLogxK

U2 - 10.1016/j.jasms.2005.07.017

DO - 10.1016/j.jasms.2005.07.017

M3 - Article

C2 - 16182559

AN - SCOPUS:26944455186

VL - 16

SP - 1805

EP - 1811

JO - Journal of the American Society for Mass Spectrometry

JF - Journal of the American Society for Mass Spectrometry

SN - 1044-0305

IS - 11

ER -