Sensitive LC MS quantitative analysis of carbohydrates by Cs+ attachment

Eduard Rogatsky, Harsha Jayatillake, Gayotri Goswami, Vlad Tomuta, Daniel Stein

Research output: Contribution to journalArticle

46 Scopus citations

Abstract

The development of a sensitive assay for the quantitative analysis of carbohydrates from human plasma using LC/MS/MS is described in this paper. After sample preparation, carbohydrates were cationized by Cs+ after their separation by normal phase liquid chromatography on an amino based column. Cesium is capable of forming a quasi-molecular ion [M + Cs]+ with neutral carbohydrate molecules in the positive ion mode of electrospray ionization mass spectrometry. The mass spectrometer was operated in multiple reaction monitoring mode, and transitions [M + 133] → 133 were monitored (M, carbohydrate molecular weight). The new method is robust, highly sensitive, rapid, and does not require postcolumn addition or derivatization. It is useful in clinical research for measurement of carbohydrate molecules by isotope dilution assay.

Original languageEnglish (US)
Pages (from-to)1805-1811
Number of pages7
JournalJournal of the American Society for Mass Spectrometry
Volume16
Issue number11
DOIs
StatePublished - Nov 1 2005

ASJC Scopus subject areas

  • Structural Biology
  • Spectroscopy

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