Self-recognition of CD1 by γ/δ T cells: Implications for innate immunity

Franca M. Spada, Ethan P. Grant, Peter J. Peters, Masahiko Sugita, Augustín Melián, David S. Leslie, Hoi K. Lee, Elly Van Donselaar, Dennis A. Hanson, Alan M. Krensky, Otto Majdic, Steven A. Porcelli, Craig T. Morita, Michael B. Brenner

Research output: Contribution to journalArticle

289 Citations (Scopus)

Abstract

The specificity of immunoglobulins and α/β T cell receptors (TCRs) provides a framework for the molecular basis of antigen recognition. Yet, evolution has preserved a separate lineage of γ/δ antigen receptors that share characteristics of both immunoglobulins and α/β TCRs but whose antigens remain poorly understood. We now show that T cells of the major tissue γ/δ T cell subset recognize nonpolymorphic CD1c molecules. These T cells proliferated in response to CD1+ presenter cells, lysed CD1c+ targets, and released T helper type 1 (Th1) cytokines. The CD1c-reactive γ/δ T cells were cytotoxic and used both perforin- and Fas-mediated cytotoxicity. Moreover, they produced granulysin, an important antimicrobial protein. Recognition of CD1c was TCR mediated, as recognition was transferred by transfection of the γ/δ TCR. Importantly, all CD1c-reactive γ/δ T cells express Vδ1 TCRs, the TCR expressed by most tissue γ/δ T cells. Recognition by this tissue pool of γ/δ T cells provides the human immune system with the capacity to respond rapidly to nonpolymorphic molecules on professional antigen presenting cells (APCs) in the absence of foreign antigens that may activate or eliminate the APCs. The presence of bactericidal granulysin suggests these cells may directly mediate host defense even before foreign antigen-specific T cells have differentiated.

Original languageEnglish (US)
Pages (from-to)937-948
Number of pages12
JournalJournal of Experimental Medicine
Volume191
Issue number6
DOIs
StatePublished - Mar 20 2000
Externally publishedYes

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Innate Immunity
T-Cell Antigen Receptor
T-Lymphocytes
Antigen-Presenting Cells
Antigens
Immunoglobulins
T-Cell Antigen Receptor Specificity
Perforin
Antigen Receptors
T-Lymphocyte Subsets
Transfection
Immune System
Cytokines
Proteins

Keywords

  • CD1
  • Cytolysis
  • Granulysin
  • T cell antigen receptors γ/δ
  • T lymphocytes

ASJC Scopus subject areas

  • Immunology

Cite this

Spada, F. M., Grant, E. P., Peters, P. J., Sugita, M., Melián, A., Leslie, D. S., ... Brenner, M. B. (2000). Self-recognition of CD1 by γ/δ T cells: Implications for innate immunity. Journal of Experimental Medicine, 191(6), 937-948. https://doi.org/10.1084/jem.191.6.937

Self-recognition of CD1 by γ/δ T cells : Implications for innate immunity. / Spada, Franca M.; Grant, Ethan P.; Peters, Peter J.; Sugita, Masahiko; Melián, Augustín; Leslie, David S.; Lee, Hoi K.; Van Donselaar, Elly; Hanson, Dennis A.; Krensky, Alan M.; Majdic, Otto; Porcelli, Steven A.; Morita, Craig T.; Brenner, Michael B.

In: Journal of Experimental Medicine, Vol. 191, No. 6, 20.03.2000, p. 937-948.

Research output: Contribution to journalArticle

Spada, FM, Grant, EP, Peters, PJ, Sugita, M, Melián, A, Leslie, DS, Lee, HK, Van Donselaar, E, Hanson, DA, Krensky, AM, Majdic, O, Porcelli, SA, Morita, CT & Brenner, MB 2000, 'Self-recognition of CD1 by γ/δ T cells: Implications for innate immunity', Journal of Experimental Medicine, vol. 191, no. 6, pp. 937-948. https://doi.org/10.1084/jem.191.6.937
Spada FM, Grant EP, Peters PJ, Sugita M, Melián A, Leslie DS et al. Self-recognition of CD1 by γ/δ T cells: Implications for innate immunity. Journal of Experimental Medicine. 2000 Mar 20;191(6):937-948. https://doi.org/10.1084/jem.191.6.937
Spada, Franca M. ; Grant, Ethan P. ; Peters, Peter J. ; Sugita, Masahiko ; Melián, Augustín ; Leslie, David S. ; Lee, Hoi K. ; Van Donselaar, Elly ; Hanson, Dennis A. ; Krensky, Alan M. ; Majdic, Otto ; Porcelli, Steven A. ; Morita, Craig T. ; Brenner, Michael B. / Self-recognition of CD1 by γ/δ T cells : Implications for innate immunity. In: Journal of Experimental Medicine. 2000 ; Vol. 191, No. 6. pp. 937-948.
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