Self-administered home cervicovaginal lavage: A novel tool for the clinical-epidemiologic investigation of genital human papillomavirus infections

E. A B Morrison, G. L. Goldberg, R. J. Hagan, A. S. Kadish, Robert D. Burk

Research output: Contribution to journalArticle

44 Citations (Scopus)

Abstract

Objective: Our objective was to evaluate a self-administered cervicovaginal irrigation apparatus (MY-PAP, Medtech, Bohemia, N.Y.) for the detection of cervicovaginal human papillomavirus infection. Study design: Twenty-five women referred to a colposcopy clinic because of a recent abnormal Papanicolaou smear were studied. Human papillomavirus infection test results detected by Southern blot hybridization and polymerase chain reaction from physician-administered and MY-PAP self-administered cervicovaginal lavage were paired for comparison. Results: Seventeen (68%) patients returned home samples by mail a mean of 13 days after the clinic visit. All clinic samples and 16 of 17 (94%) home samples yielded adequate deoxyribonucleic acid for human papillomavirus infection analyses. Human papillomavirus infection was detected by polymerase chain reaction in 14 of 16 (88%) samples with complete concordance of viral detection in paired samples from the clinic and home. Southern blot detected human papillomavirus infection in 13 of 25 clinic samples (52%) and 7 of 16 home samples (44%), with concordance in 12 of 16 paired samples (75%). Specific viral type was the same in all positive paired samples (6/6). Concordance was high when the initial sample had a strong (2+ to 4+) human papilloma virus infection signal (5/6, 83%) or a negative result (5/6) or when a dysplastic (cervical intraepithelial neoplasia grade 1 to 3) lesion (9/11, 82%) was seen on cervical biopsy. Conclusion: Self-administered lavage represents an extremely promising technique for obtaining cervicovaginal lavage samples for human papillomavirus infection analyses.

Original languageEnglish (US)
Pages (from-to)104-107
Number of pages4
JournalAmerican Journal of Obstetrics and Gynecology
Volume167
Issue number1
StatePublished - 1992

Fingerprint

Papillomavirus Infections
Therapeutic Irrigation
Southern Blotting
Papillomaviridae
Papanicolaou Test
Matched-Pair Analysis
Polymerase Chain Reaction
Colposcopy
Cervical Intraepithelial Neoplasia
Postal Service
Virus Diseases
Ambulatory Care
Physicians
Biopsy
DNA

Keywords

  • cervicovaginal lavage
  • human papillomavirus infection
  • polymerase chain reaction
  • Southern blot

ASJC Scopus subject areas

  • Medicine(all)
  • Obstetrics and Gynecology

Cite this

Self-administered home cervicovaginal lavage : A novel tool for the clinical-epidemiologic investigation of genital human papillomavirus infections. / Morrison, E. A B; Goldberg, G. L.; Hagan, R. J.; Kadish, A. S.; Burk, Robert D.

In: American Journal of Obstetrics and Gynecology, Vol. 167, No. 1, 1992, p. 104-107.

Research output: Contribution to journalArticle

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