TY - JOUR
T1 - Self-administered home cervicovaginal lavage
T2 - A novel tool for the clinical-epidemiologic investigation of genital human papillomavirus infections
AU - Morrison, Ellen A.B.
AU - Goldberg, Gary L.
AU - Hagan, Robert J.
AU - Kadish, Anna S.
AU - Burk, Robert D.
N1 - Funding Information:
From the Departments of Medicine,' Obstetrics and Gynecology,' Pathology,' Pediatrics,d and Microbiology and Immunology,' Albert Einstein College of Medicine. Supported in part by National Institutes of Health, National Institute of Allergy and Infectious Diseases training grant AI07183-12 and National Institutes of Health grants 5P30CA13330 and CA47630. Dr. Burk was the recipient of a Faculty Award from the American Cancer Society. Presented in part at the International Papillomavirus Workshop, Seattle, Washington, July 20-26, 1991. Received for publication October 30, 1991; revised January 27, 1992; accepted January 31,1992. Reprint requests: Robert D. Burh, MD, Albert Einstein College of Medicine, 1300 Morris Parh Ave., Bronx, NY 10461,
PY - 1992
Y1 - 1992
N2 - Objective: Our objective was to evaluate a self-administered cervicovaginal irrigation apparatus (MY-PAP, Medtech, Bohemia, N.Y.) for the detection of cervicovaginal human papillomavirus infection. Study Design: Twenty-five women referred to a colposcopy clinic because of a recent abnormal Papanicolaou smear were studied. Human papillomavirus infection test results detected by Southern blot hybridization and polymerase chain reaction from physician-administered and MY-PAP self-administered cervicovaginal lavage were paired for comparison. Results: Seventeen (68%) patients returned home samples by mail a mean of 13 days after the clinic visit. All clinic samples and 16 of 17 (94%) home samples yielded adequate deoxyribonucleic acid for human papillomavirus infection analyses. Human papillomavirus infection was detected by polymerase chain reaction in 14 of 16 (88%) samples with complete concordance of viral detection in paired samples from the clinic and home. Southern blot detected human papillomavirus infection in 13 of 25 clinic samples (52%) and 7 of 16 home samples (44%), with concordance in 12 of 16 paired samples (75%). Specific viral type was the same in all positive paired samples (6/6). Concordance was high when the initial sample had a strong (2+ to 4 + ) human papillomavirus infection signal (5/6, 83%) or a negative result (5/6) or when a dysplastic (cervical intraepithelial neoplasia grade 1 to 3) lesion (9/11, 82%) was seen on cervical biopsy. Conclusion: Self-administered lavage represents an extremely promising technique for obtaining cervicovaginal lavage samples for human papillomavirus infection analyses.
AB - Objective: Our objective was to evaluate a self-administered cervicovaginal irrigation apparatus (MY-PAP, Medtech, Bohemia, N.Y.) for the detection of cervicovaginal human papillomavirus infection. Study Design: Twenty-five women referred to a colposcopy clinic because of a recent abnormal Papanicolaou smear were studied. Human papillomavirus infection test results detected by Southern blot hybridization and polymerase chain reaction from physician-administered and MY-PAP self-administered cervicovaginal lavage were paired for comparison. Results: Seventeen (68%) patients returned home samples by mail a mean of 13 days after the clinic visit. All clinic samples and 16 of 17 (94%) home samples yielded adequate deoxyribonucleic acid for human papillomavirus infection analyses. Human papillomavirus infection was detected by polymerase chain reaction in 14 of 16 (88%) samples with complete concordance of viral detection in paired samples from the clinic and home. Southern blot detected human papillomavirus infection in 13 of 25 clinic samples (52%) and 7 of 16 home samples (44%), with concordance in 12 of 16 paired samples (75%). Specific viral type was the same in all positive paired samples (6/6). Concordance was high when the initial sample had a strong (2+ to 4 + ) human papillomavirus infection signal (5/6, 83%) or a negative result (5/6) or when a dysplastic (cervical intraepithelial neoplasia grade 1 to 3) lesion (9/11, 82%) was seen on cervical biopsy. Conclusion: Self-administered lavage represents an extremely promising technique for obtaining cervicovaginal lavage samples for human papillomavirus infection analyses.
KW - Cervicovaginal lavage
KW - Southern blot
KW - human papillomavirus infection
KW - polymerase chain reaction
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U2 - 10.1016/S0002-9378(11)91637-8
DO - 10.1016/S0002-9378(11)91637-8
M3 - Article
C2 - 1332474
AN - SCOPUS:0026633545
SN - 0002-9378
VL - 167
SP - 104
EP - 107
JO - American journal of obstetrics and gynecology
JF - American journal of obstetrics and gynecology
IS - 1
ER -