Seleno- and Telluro-xylofuranosides attenuate Mn-induced toxicity in C. elegans via the DAF-16/FOXO pathway

Suzi G N Wollenhaupt, Ana Thalita Soares, Willian G. Salgueiro, Simone Noremberg, Gabriel Reis, Carine Viana, Priscila Gubert, Felix A. Soares, Ricardo F. Affeldt, Diogo S. Lüdtke, Francielli W. Santos, Cristiane C. Denardin, Michael Aschner, Daiana S. Avila

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

Organochalcogens are promising pharmacological agents that possess significant biological activities. Nevertheless, because of the complexity of mammalian models, it has been difficult to determine the molecular pathways and specific proteins that are modulated in response to treatments with these compounds. The nematode worm Caenorhabditis elegans is an alternative experimental model that affords easy genetic manipulations, green fluorescent protein tagging and in vivo live analysis of toxicity. Abundant evidence points to oxidative stress in mediating manganese (Mn)-induced toxicity. In this study we challenged worms with Mn, and investigated the efficacy of inedited selenium- and tellurium-xylofuranosides in reversing and/or protecting the worms from Mn-induced toxicity. In addition, we investigated their putative mechanism of action. First, we determined the lethal dose 50% (LD50) and the effects of the xylofuranosides on various toxic parameters. This was followed by studies on the ability of xylofuranosides to afford protection against Mn-induced toxicity. Both Se- and Te-xylofuranosides increased the expression of superoxide dismutase (SOD-3). Furthermore, we observed that the xylofuranosides induced nuclear translocation of the transcription factor DAF-16/FOXO, which in the worm is known to regulate stress responsiveness, aging and metabolism. These findings suggest that xylofuranosides attenuate toxicity Mn-induced, by regulating the DAF-16/FOXO signaling pathway.

Original languageEnglish (US)
Pages (from-to)192-199
Number of pages8
JournalFood and Chemical Toxicology
Volume64
DOIs
StatePublished - 2014
Externally publishedYes

Fingerprint

Manganese
manganese
Toxicity
toxicity
superoxide dismutase
tellurium
Tellurium
Oxidative stress
Poisons
Lethal Dose 50
Caenorhabditis elegans
Selenium
Green Fluorescent Proteins
Bioactivity
lethal dose 50
green fluorescent protein
genetic engineering
Metabolism
selenium
Superoxide Dismutase

Keywords

  • C. elegans
  • DAF-16
  • Manganese
  • Oxidative stress
  • SOD
  • Xylofuranoside

ASJC Scopus subject areas

  • Toxicology
  • Food Science

Cite this

Wollenhaupt, S. G. N., Soares, A. T., Salgueiro, W. G., Noremberg, S., Reis, G., Viana, C., ... Avila, D. S. (2014). Seleno- and Telluro-xylofuranosides attenuate Mn-induced toxicity in C. elegans via the DAF-16/FOXO pathway. Food and Chemical Toxicology, 64, 192-199. https://doi.org/10.1016/j.fct.2013.11.030

Seleno- and Telluro-xylofuranosides attenuate Mn-induced toxicity in C. elegans via the DAF-16/FOXO pathway. / Wollenhaupt, Suzi G N; Soares, Ana Thalita; Salgueiro, Willian G.; Noremberg, Simone; Reis, Gabriel; Viana, Carine; Gubert, Priscila; Soares, Felix A.; Affeldt, Ricardo F.; Lüdtke, Diogo S.; Santos, Francielli W.; Denardin, Cristiane C.; Aschner, Michael; Avila, Daiana S.

In: Food and Chemical Toxicology, Vol. 64, 2014, p. 192-199.

Research output: Contribution to journalArticle

Wollenhaupt, SGN, Soares, AT, Salgueiro, WG, Noremberg, S, Reis, G, Viana, C, Gubert, P, Soares, FA, Affeldt, RF, Lüdtke, DS, Santos, FW, Denardin, CC, Aschner, M & Avila, DS 2014, 'Seleno- and Telluro-xylofuranosides attenuate Mn-induced toxicity in C. elegans via the DAF-16/FOXO pathway', Food and Chemical Toxicology, vol. 64, pp. 192-199. https://doi.org/10.1016/j.fct.2013.11.030
Wollenhaupt, Suzi G N ; Soares, Ana Thalita ; Salgueiro, Willian G. ; Noremberg, Simone ; Reis, Gabriel ; Viana, Carine ; Gubert, Priscila ; Soares, Felix A. ; Affeldt, Ricardo F. ; Lüdtke, Diogo S. ; Santos, Francielli W. ; Denardin, Cristiane C. ; Aschner, Michael ; Avila, Daiana S. / Seleno- and Telluro-xylofuranosides attenuate Mn-induced toxicity in C. elegans via the DAF-16/FOXO pathway. In: Food and Chemical Toxicology. 2014 ; Vol. 64. pp. 192-199.
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AU - Wollenhaupt, Suzi G N

AU - Soares, Ana Thalita

AU - Salgueiro, Willian G.

AU - Noremberg, Simone

AU - Reis, Gabriel

AU - Viana, Carine

AU - Gubert, Priscila

AU - Soares, Felix A.

AU - Affeldt, Ricardo F.

AU - Lüdtke, Diogo S.

AU - Santos, Francielli W.

AU - Denardin, Cristiane C.

AU - Aschner, Michael

AU - Avila, Daiana S.

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N2 - Organochalcogens are promising pharmacological agents that possess significant biological activities. Nevertheless, because of the complexity of mammalian models, it has been difficult to determine the molecular pathways and specific proteins that are modulated in response to treatments with these compounds. The nematode worm Caenorhabditis elegans is an alternative experimental model that affords easy genetic manipulations, green fluorescent protein tagging and in vivo live analysis of toxicity. Abundant evidence points to oxidative stress in mediating manganese (Mn)-induced toxicity. In this study we challenged worms with Mn, and investigated the efficacy of inedited selenium- and tellurium-xylofuranosides in reversing and/or protecting the worms from Mn-induced toxicity. In addition, we investigated their putative mechanism of action. First, we determined the lethal dose 50% (LD50) and the effects of the xylofuranosides on various toxic parameters. This was followed by studies on the ability of xylofuranosides to afford protection against Mn-induced toxicity. Both Se- and Te-xylofuranosides increased the expression of superoxide dismutase (SOD-3). Furthermore, we observed that the xylofuranosides induced nuclear translocation of the transcription factor DAF-16/FOXO, which in the worm is known to regulate stress responsiveness, aging and metabolism. These findings suggest that xylofuranosides attenuate toxicity Mn-induced, by regulating the DAF-16/FOXO signaling pathway.

AB - Organochalcogens are promising pharmacological agents that possess significant biological activities. Nevertheless, because of the complexity of mammalian models, it has been difficult to determine the molecular pathways and specific proteins that are modulated in response to treatments with these compounds. The nematode worm Caenorhabditis elegans is an alternative experimental model that affords easy genetic manipulations, green fluorescent protein tagging and in vivo live analysis of toxicity. Abundant evidence points to oxidative stress in mediating manganese (Mn)-induced toxicity. In this study we challenged worms with Mn, and investigated the efficacy of inedited selenium- and tellurium-xylofuranosides in reversing and/or protecting the worms from Mn-induced toxicity. In addition, we investigated their putative mechanism of action. First, we determined the lethal dose 50% (LD50) and the effects of the xylofuranosides on various toxic parameters. This was followed by studies on the ability of xylofuranosides to afford protection against Mn-induced toxicity. Both Se- and Te-xylofuranosides increased the expression of superoxide dismutase (SOD-3). Furthermore, we observed that the xylofuranosides induced nuclear translocation of the transcription factor DAF-16/FOXO, which in the worm is known to regulate stress responsiveness, aging and metabolism. These findings suggest that xylofuranosides attenuate toxicity Mn-induced, by regulating the DAF-16/FOXO signaling pathway.

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