Selective protection of specific DNA sequences in the heterochromatin of C-banded human Y chromosomes

E. S. Cantu, R. D. Marsh, K. E. Boecklen, Harry Ostrer

Research output: Contribution to journalArticle

Abstract

The molecular basis of C-banding was investigated by in situ hybridization of human Y chromosome-derived repeated sequences, DYZ1 and DYZ2, to untreated or to alkaline-treated metaphases. Autoradiography of G-banded metaphases showed that both probes hybridized to the long arm of Y. Alkaline hydrolysis significantly reduced grain number for DYZ2 (58%-82%; P < .05) but not for DYZ1 (P > .05). Similar results were observed for interphase nuclei. These findings demonstrated that the heterochromatin of the long arm contains at least two repetitive DNA fractions having two different sensitivities to alkaline hydrolysis. These observations support the notion that DYZ2 maps terminally on the Yq arm and may be nonheterochromatic.

Original languageEnglish (US)
Pages (from-to)948-953
Number of pages6
JournalAmerican Journal of Human Genetics
Volume43
Issue number6
StatePublished - 1988
Externally publishedYes

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Chromosomes, Human, Y
Heterochromatin
Metaphase
Hydrolysis
Interphase
Autoradiography
In Situ Hybridization
DNA

ASJC Scopus subject areas

  • Genetics

Cite this

Selective protection of specific DNA sequences in the heterochromatin of C-banded human Y chromosomes. / Cantu, E. S.; Marsh, R. D.; Boecklen, K. E.; Ostrer, Harry.

In: American Journal of Human Genetics, Vol. 43, No. 6, 1988, p. 948-953.

Research output: Contribution to journalArticle

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