Selective diapedesis of Th1 cells induced by endothelial cell RANTES

Toshihisa Kawai, Makoto Seki, Kenji Hiromatsu, Jean W. Eastcott, Gerald F M Watts, Motoyuki Sugai, Daniel J. Smith, Steven A. Porcelli, Martin A. Taubman

Research output: Contribution to journalArticle

101 Citations (Scopus)

Abstract

Differentiated CD4 T cells can be divided into Th1 and Th2 types based on the cytokines they produce. Differential expression of chemokine receptors on either the Th1-type or the Th2-type cell suggests that Th1-type and Th2- type cells differ not only in cytokine production but also in their migratory capacity. Stimulation of endothelial cells with IFN-γ selectively enhanced transmigration of Th1-type cells, but not Th2-type cells, in a transendothelial migration assay. Enhanced transmigration of Th1-type cells was dependent on the chemokine RANTES produced by endothelial cells, as indicated by the findings that Ab neutralizing RANTES, or Ab to its receptor CCR5, inhibited transmigration. Neutralizing Ab to chemokines macrophage- inflammatory protein-1α or monocyte chemotactic protein-1 did not inhibit Th1 selective migration. Whereas anti-CD18 and anti-CD54 blocked basal levels of Th1-type cell adherence to endothelial cells and also inhibited transmigration, anti-RANTES blocked only transmigration, indicating that RANTES appeared to induce transmigration of adherent T cells. RANTES seemed to promote diapedesis of adherent Th1-type cells by augmenting pseudopod formation in conjunction with actin rearrangement by a pathway that was sensitive to the phosphoinositol 3-kinase inhibitor wortmannin and to the Rho GTP-binding protein inhibitor, epidermal cell differentiation inhibitor. Thus, enhancement of Th1-type selective migration appeared to be responsible for the diapedesis induced by interaction between CCR5 on Th1-type cells and RANTES produced by endothelial cells. Further evidence that CCR5 and RANTES play a modulatory role in Th1-type selective migration derives from the abrogation of this migration by anti-RANTES and anti-CCR5 Abs.

Original languageEnglish (US)
Pages (from-to)3269-3278
Number of pages10
JournalJournal of Immunology
Volume163
Issue number6
StatePublished - Sep 15 1999
Externally publishedYes

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Transendothelial and Transepithelial Migration
Chemokine CCL5
Th1 Cells
Endothelial Cells
Th2 Cells
Chemokines
CCR5 Receptors
Cytokines
T-Lymphocytes
Macrophage Inflammatory Proteins
rho GTP-Binding Proteins
Pseudopodia
Chemokine CCL2
Chemokine Receptors
Actins
Cell Differentiation
Phosphotransferases

ASJC Scopus subject areas

  • Immunology

Cite this

Kawai, T., Seki, M., Hiromatsu, K., Eastcott, J. W., Watts, G. F. M., Sugai, M., ... Taubman, M. A. (1999). Selective diapedesis of Th1 cells induced by endothelial cell RANTES. Journal of Immunology, 163(6), 3269-3278.

Selective diapedesis of Th1 cells induced by endothelial cell RANTES. / Kawai, Toshihisa; Seki, Makoto; Hiromatsu, Kenji; Eastcott, Jean W.; Watts, Gerald F M; Sugai, Motoyuki; Smith, Daniel J.; Porcelli, Steven A.; Taubman, Martin A.

In: Journal of Immunology, Vol. 163, No. 6, 15.09.1999, p. 3269-3278.

Research output: Contribution to journalArticle

Kawai, T, Seki, M, Hiromatsu, K, Eastcott, JW, Watts, GFM, Sugai, M, Smith, DJ, Porcelli, SA & Taubman, MA 1999, 'Selective diapedesis of Th1 cells induced by endothelial cell RANTES', Journal of Immunology, vol. 163, no. 6, pp. 3269-3278.
Kawai T, Seki M, Hiromatsu K, Eastcott JW, Watts GFM, Sugai M et al. Selective diapedesis of Th1 cells induced by endothelial cell RANTES. Journal of Immunology. 1999 Sep 15;163(6):3269-3278.
Kawai, Toshihisa ; Seki, Makoto ; Hiromatsu, Kenji ; Eastcott, Jean W. ; Watts, Gerald F M ; Sugai, Motoyuki ; Smith, Daniel J. ; Porcelli, Steven A. ; Taubman, Martin A. / Selective diapedesis of Th1 cells induced by endothelial cell RANTES. In: Journal of Immunology. 1999 ; Vol. 163, No. 6. pp. 3269-3278.
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AU - Sugai, Motoyuki

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