Selective deficiency of guanine nucleotide-binding protein G(o) in two dopamine-resistant pituitary tumors

R. Collu, C. Bouvier, G. Lagace, C. G. Unson, G. Milligan, P. Goldsmith, Allen M. Spiegel

Research output: Contribution to journalArticle

41 Citations (Scopus)

Abstract

G proteins were quantitated by immunoblot in normal rat anterior pituitary, an estrone-induced pituitary adenoma, and in two transplantable pituitary tumors resistant to dopamine, 7315a and MtTW15. Antisera specific for the α(o) and β subunits or the α(i) subunit of G proteins were tested with all preparations. While the α(i) and β subunits were found to be present in variable concentrations in all preparations, the α(o) subunit was very low or undetectable in the transplantable tumors. These findings suggest that tumor resistance to the dopamine inhibitory actions on prolactin release and on tumor growth may be due to the deficiency of a G(o) protein.

Original languageEnglish (US)
Pages (from-to)1176-1178
Number of pages3
JournalEndocrinology
Volume122
Issue number3
StatePublished - 1988
Externally publishedYes

Fingerprint

Guanine Nucleotides
Pituitary Neoplasms
Dopamine
Carrier Proteins
GTP-Binding Proteins
Gi-Go GTP-Binding Protein alpha Subunits
Neoplasms
Estrone
Prolactin
Immune Sera
Growth

ASJC Scopus subject areas

  • Endocrinology
  • Endocrinology, Diabetes and Metabolism

Cite this

Collu, R., Bouvier, C., Lagace, G., Unson, C. G., Milligan, G., Goldsmith, P., & Spiegel, A. M. (1988). Selective deficiency of guanine nucleotide-binding protein G(o) in two dopamine-resistant pituitary tumors. Endocrinology, 122(3), 1176-1178.

Selective deficiency of guanine nucleotide-binding protein G(o) in two dopamine-resistant pituitary tumors. / Collu, R.; Bouvier, C.; Lagace, G.; Unson, C. G.; Milligan, G.; Goldsmith, P.; Spiegel, Allen M.

In: Endocrinology, Vol. 122, No. 3, 1988, p. 1176-1178.

Research output: Contribution to journalArticle

Collu, R, Bouvier, C, Lagace, G, Unson, CG, Milligan, G, Goldsmith, P & Spiegel, AM 1988, 'Selective deficiency of guanine nucleotide-binding protein G(o) in two dopamine-resistant pituitary tumors', Endocrinology, vol. 122, no. 3, pp. 1176-1178.
Collu R, Bouvier C, Lagace G, Unson CG, Milligan G, Goldsmith P et al. Selective deficiency of guanine nucleotide-binding protein G(o) in two dopamine-resistant pituitary tumors. Endocrinology. 1988;122(3):1176-1178.
Collu, R. ; Bouvier, C. ; Lagace, G. ; Unson, C. G. ; Milligan, G. ; Goldsmith, P. ; Spiegel, Allen M. / Selective deficiency of guanine nucleotide-binding protein G(o) in two dopamine-resistant pituitary tumors. In: Endocrinology. 1988 ; Vol. 122, No. 3. pp. 1176-1178.
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