Selective control of basolateral membrane protein polarity by cdc42

David Cohen, Anne Muesch, Enrique Rodriguez-Boulan

Research output: Contribution to journalArticle

56 Citations (Scopus)

Abstract

The rho GTPase cdc42 is implicated in several aspects of cell polarity. A recent study (Kroschewski R, Hall A, Mellman I. Nat Cell Biol 1999;1:8-13) demonstrated that a dominant negative mutant of cdc42 abolishes the polarity of basolateral membrane proteins in MDCK cells, but did not elucidate whether this effect was selective for basolateral proteins or nonselective for all secreted proteins. To answer this question, we analyzed the polarity of newly synthesized membrane and soluble proteins in MDCK cell lines previously induced to overexpress mutant forms of cdc42. GTPase-deficient and dominant negative cdc42 did not affect the apical targeting of a newly synthesized apical membrane protein, but reversed to apical the distribution of two exogenous basolateral membrane proteins. In striking contrast, GTPase-deficient cdc42 did not affect polarized exocytosis of endogenous soluble proteins, either apical or basolateral. The exquisitely selective regulation of polarized protein targeting by cdc42 may allow cells to fine-tune their membrane composition in response to extracellular signals during development, migration and in response to injury.

Original languageEnglish (US)
Pages (from-to)556-564
Number of pages9
JournalTraffic
Volume2
Issue number8
DOIs
StatePublished - 2001
Externally publishedYes

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Membrane Proteins
Madin Darby Canine Kidney Cells
GTP Phosphohydrolases
Proteins
rho GTP-Binding Proteins
Cell Polarity
Exocytosis
Membranes
Protein Transport
Cell Line
Cells
Wounds and Injuries
Chemical analysis

Keywords

  • Cdc42
  • Polarity
  • Protein targeting

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology
  • Structural Biology
  • Molecular Biology
  • Genetics

Cite this

Selective control of basolateral membrane protein polarity by cdc42. / Cohen, David; Muesch, Anne; Rodriguez-Boulan, Enrique.

In: Traffic, Vol. 2, No. 8, 2001, p. 556-564.

Research output: Contribution to journalArticle

Cohen, David ; Muesch, Anne ; Rodriguez-Boulan, Enrique. / Selective control of basolateral membrane protein polarity by cdc42. In: Traffic. 2001 ; Vol. 2, No. 8. pp. 556-564.
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