Selection of deoxyribozyme ligases that catalyze the formation of an unnatural internucleotide linkage

M. Levy, A. D. Ellington

Research output: Contribution to journalArticle

26 Scopus citations

Abstract

The chemical ligation of DNA molecules can be mediated by terminal phosphorothioate displacement of a 5′ iodine. We have selected deoxyribozymes that can catalyze the formation of such phosphorothioester internucleotide linkages. The selected deoxyribozymes enhance the rate of ligation in part through the provision of a template that aligns the ligation junction and do not appear to require metal ions for catalysis.

Original languageEnglish (US)
Pages (from-to)2581-2587
Number of pages7
JournalBioorganic and Medicinal Chemistry
Volume9
Issue number10
DOIs
StatePublished - Sep 17 2001

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Medicine
  • Molecular Biology
  • Pharmaceutical Science
  • Drug Discovery
  • Clinical Biochemistry
  • Organic Chemistry

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