Selection of deoxyribozyme ligases that catalyze the formation of an unnatural internucleotide linkage

M. Levy, A. D. Ellington

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

The chemical ligation of DNA molecules can be mediated by terminal phosphorothioate displacement of a 5′ iodine. We have selected deoxyribozymes that can catalyze the formation of such phosphorothioester internucleotide linkages. The selected deoxyribozymes enhance the rate of ligation in part through the provision of a template that aligns the ligation junction and do not appear to require metal ions for catalysis.

Original languageEnglish (US)
Pages (from-to)2581-2587
Number of pages7
JournalBioorganic and Medicinal Chemistry
Volume9
Issue number10
DOIs
StatePublished - 2001
Externally publishedYes

Fingerprint

Catalytic DNA
Ligases
Ligation
Iodine
Catalysis
Metal ions
Molecules
DNA
Metals
Ions

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Organic Chemistry
  • Drug Discovery
  • Pharmaceutical Science

Cite this

Selection of deoxyribozyme ligases that catalyze the formation of an unnatural internucleotide linkage. / Levy, M.; Ellington, A. D.

In: Bioorganic and Medicinal Chemistry, Vol. 9, No. 10, 2001, p. 2581-2587.

Research output: Contribution to journalArticle

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