Abstract
The chemical ligation of DNA molecules can be mediated by terminal phosphorothioate displacement of a 5′ iodine. We have selected deoxyribozymes that can catalyze the formation of such phosphorothioester internucleotide linkages. The selected deoxyribozymes enhance the rate of ligation in part through the provision of a template that aligns the ligation junction and do not appear to require metal ions for catalysis.
Original language | English (US) |
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Pages (from-to) | 2581-2587 |
Number of pages | 7 |
Journal | Bioorganic and Medicinal Chemistry |
Volume | 9 |
Issue number | 10 |
DOIs | |
State | Published - 2001 |
Externally published | Yes |
ASJC Scopus subject areas
- Biochemistry
- Molecular Medicine
- Molecular Biology
- Pharmaceutical Science
- Drug Discovery
- Clinical Biochemistry
- Organic Chemistry