TY - JOUR
T1 - Secretion of nuclease across the outer membrane of Serratia marcescens and its energy requirements
AU - Suh, Yousin
AU - Benedik, Michael J.
PY - 1997
Y1 - 1997
N2 - Extracellular secretion of Serratia marcescens nuclease occurs as a two- step process via a periplasmic intermediate. Unlike other extracellular proteins secreted by gram-negative bacteria by the general secretory pathway, nuclease accumulates in the periplasm in its active form for an unusually long time before its export into the growth medium. The energy requirements for extracellular secretion of nuclease from the periplasm were investigated. Our results suggest that the second step of secretion across the outer membrane is dependent upon the external pH; acidic pH effectively but reversibly blocks extracellular secretion. However, electrochemical proton gradient, and possibly ATP hydrolysis, are not required for this step. We suggest that nuclease uses a novel mechanism for the second step of secretion in S. marcescens.
AB - Extracellular secretion of Serratia marcescens nuclease occurs as a two- step process via a periplasmic intermediate. Unlike other extracellular proteins secreted by gram-negative bacteria by the general secretory pathway, nuclease accumulates in the periplasm in its active form for an unusually long time before its export into the growth medium. The energy requirements for extracellular secretion of nuclease from the periplasm were investigated. Our results suggest that the second step of secretion across the outer membrane is dependent upon the external pH; acidic pH effectively but reversibly blocks extracellular secretion. However, electrochemical proton gradient, and possibly ATP hydrolysis, are not required for this step. We suggest that nuclease uses a novel mechanism for the second step of secretion in S. marcescens.
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U2 - 10.1128/jb.179.3.677-683.1997
DO - 10.1128/jb.179.3.677-683.1997
M3 - Article
C2 - 9006020
AN - SCOPUS:0031028560
SN - 0021-9193
VL - 179
SP - 677
EP - 683
JO - Journal of Bacteriology
JF - Journal of Bacteriology
IS - 3
ER -