TY - JOUR
T1 - Secreted lipases supply fatty acids for yeast growth in the absence of de novo fatty acid synthesis
AU - Nguyen, Long Nam
AU - Gacser, Attila
AU - Nosanchuk, Joshua D.
N1 - Funding Information:
J.D.N. is supported in part by an Irma T. Hirschl/Monique Weill-Caulier Trust Research Award. A.G. is supported in part by a Hungarian Scientific Research Fund (PD 73250 and NF 84006), EMBO Installation Grant and János Bolyai Research Scholarship of the Hungarian Academy of Sciences.
PY - 2011
Y1 - 2011
N2 - The yeast Candida parapsilosis has emerged as a major human pathogen. The fungus is found in diverse environments as well as in different mammalian hosts, indicative of a successful adaptation to various niches. Fatty acids are the building blocks of cell membranes. Thus, the yeast must have evolved efficient ways to assimilate fatty acids from different sources, such as glucose via de novo fatty acid synthesis or lipids via lipolysis. We have recently shown that blocking the fatty acid synthesis pathway or interfering with the production of secreted lipases impeded yeast growth in glucose and lipid-containing media, respectively. However, in a more complex media (e.g., presence of glucose and lipids), blockage of either pathway individually resulted in growth similar to wild-type yeast. Here, we demonstrate that dual inhibition of these pathways significantly decreased yeast growth in complex media. Therefore, we propose that simultaneously targeting secreted lipases and fatty acid pathways might be ideal to combat C. parapsilosis disease, and perhaps other pathogenic fungi.
AB - The yeast Candida parapsilosis has emerged as a major human pathogen. The fungus is found in diverse environments as well as in different mammalian hosts, indicative of a successful adaptation to various niches. Fatty acids are the building blocks of cell membranes. Thus, the yeast must have evolved efficient ways to assimilate fatty acids from different sources, such as glucose via de novo fatty acid synthesis or lipids via lipolysis. We have recently shown that blocking the fatty acid synthesis pathway or interfering with the production of secreted lipases impeded yeast growth in glucose and lipid-containing media, respectively. However, in a more complex media (e.g., presence of glucose and lipids), blockage of either pathway individually resulted in growth similar to wild-type yeast. Here, we demonstrate that dual inhibition of these pathways significantly decreased yeast growth in complex media. Therefore, we propose that simultaneously targeting secreted lipases and fatty acid pathways might be ideal to combat C. parapsilosis disease, and perhaps other pathogenic fungi.
KW - Candida
KW - Fatty acid desaturase Ole1
KW - Fatty acid synthase Fas2
KW - Fatty acid synthesis
KW - Secreted lipases
KW - Yeast growth
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U2 - 10.4161/viru.2.6.18244
DO - 10.4161/viru.2.6.18244
M3 - Article
C2 - 22030857
AN - SCOPUS:82955239963
SN - 2150-5594
VL - 2
SP - 538
EP - 541
JO - Virulence
JF - Virulence
IS - 6
ER -