Secreted lipases supply fatty acids for yeast growth in the absence of de novo fatty acid synthesis

Long Nam Nguyen, Attila Gacser, Joshua D. Nosanchuk

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

The yeast Candida parapsilosis has emerged as a major human pathogen. The fungus is found in diverse environments as well as in different mammalian hosts, indicative of a successful adaptation to various niches. Fatty acids are the building blocks of cell membranes. Thus, the yeast must have evolved efficient ways to assimilate fatty acids from different sources, such as glucose via de novo fatty acid synthesis or lipids via lipolysis. We have recently shown that blocking the fatty acid synthesis pathway or interfering with the production of secreted lipases impeded yeast growth in glucose and lipid-containing media, respectively. However, in a more complex media (e.g., presence of glucose and lipids), blockage of either pathway individually resulted in growth similar to wild-type yeast. Here, we demonstrate that dual inhibition of these pathways significantly decreased yeast growth in complex media. Therefore, we propose that simultaneously targeting secreted lipases and fatty acid pathways might be ideal to combat C. parapsilosis disease, and perhaps other pathogenic fungi.

Original languageEnglish (US)
Pages (from-to)538-541
Number of pages4
JournalVirulence
Volume2
Issue number6
StatePublished - Nov 2011

Fingerprint

Lipase
Fatty Acids
Yeasts
Growth
Lipids
Glucose
Fungi
Lipolysis
Candida
Cell Membrane

Keywords

  • Candida
  • Fatty acid desaturase Ole1
  • Fatty acid synthase Fas2
  • Fatty acid synthesis
  • Secreted lipases
  • Yeast growth

ASJC Scopus subject areas

  • Infectious Diseases
  • Microbiology
  • Parasitology
  • Immunology
  • Microbiology (medical)

Cite this

Secreted lipases supply fatty acids for yeast growth in the absence of de novo fatty acid synthesis. / Nguyen, Long Nam; Gacser, Attila; Nosanchuk, Joshua D.

In: Virulence, Vol. 2, No. 6, 11.2011, p. 538-541.

Research output: Contribution to journalArticle

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