TY - JOUR
T1 - Searching for genetic determinants of human aging and longevity
T2 - Opportunities and challenges
AU - Vijg, Jan
AU - Van Orsouw, Nathalie
N1 - Funding Information:
We thank Dr Thomas Perls and Dr Claudio Franceschi for blood samples of the centenarians. This work was supported by NIH grant CA78564, Accelerated Genomics Corporation, and CBS Scientific (the authors have a significant commercial interest in TDGS).
PY - 2002
Y1 - 2002
N2 - One way of testing possible causal relationships between various functional pathways and aging and longevity processes is to comparatively analyze groups of elderly individuals with select phenotypes for sequence variation in all genes participating in these pathways. Such direct association analysis to identify 'candidate pathways' in aging and longevity is theoretically feasible, with the complete sequence of the human genome known and massive gene annotation projects underway. To find all possible sequence variation of a large number of genes in aging populations, efficient genotyping methods are needed. Here, we describe the use of one such method, two-dimensional gene scanning (TDGS), for screening populations of centenarians and controls for polymorphic variation in the large BRCA1 breast cancer susceptibility gene.
AB - One way of testing possible causal relationships between various functional pathways and aging and longevity processes is to comparatively analyze groups of elderly individuals with select phenotypes for sequence variation in all genes participating in these pathways. Such direct association analysis to identify 'candidate pathways' in aging and longevity is theoretically feasible, with the complete sequence of the human genome known and massive gene annotation projects underway. To find all possible sequence variation of a large number of genes in aging populations, efficient genotyping methods are needed. Here, we describe the use of one such method, two-dimensional gene scanning (TDGS), for screening populations of centenarians and controls for polymorphic variation in the large BRCA1 breast cancer susceptibility gene.
KW - Aging and longevity genes
KW - BRCA1
KW - Direct association studies
KW - Single nucleotide polymorphism
KW - Two-dimensional gene scanning
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U2 - 10.1016/S0047-6374(01)00346-3
DO - 10.1016/S0047-6374(01)00346-3
M3 - Article
C2 - 11718812
AN - SCOPUS:0036132119
SN - 0047-6374
VL - 123
SP - 195
EP - 205
JO - Mechanisms of Ageing and Development
JF - Mechanisms of Ageing and Development
IS - 2-3
ER -
