Seamless Ligation Cloning Extract (SLiCE) cloning method

Research output: Chapter in Book/Report/Conference proceedingChapter

45 Scopus citations


SLiCE (Seamless Ligation Cloning Extract) is a novel cloning method that utilizes easy to generate bacterial cell extracts to assemble multiple DNA fragments into recombinant DNA molecules in a single in vitro recombination reaction. SLiCE overcomes the sequence limitations of traditional cloning methods, facilitates seamless cloning by recombining short end homologies (15-52 bp) with or without flanking heterologous sequences and provides an effective strategy for directional subcloning of DNA fragments from bacterial artificial chromosomes or other sources. SLiCE is highly cost-effective and demonstrates the versatility as a number of standard laboratory bacterial strains can serve as sources for SLiCE extract. We established a DH10B-derived E. coli strain expressing an optimized λ prophage Red recombination system, termed PPY, which facilitates SLiCE with very high efficiencies.

Original languageEnglish (US)
Title of host publicationDNA Cloning and Assembly Methods
PublisherHumana Press Inc.
Number of pages10
ISBN (Print)9781627037631
StatePublished - 2014
Externally publishedYes

Publication series

NameMethods in Molecular Biology
ISSN (Print)1064-3745


  • Bacterial cell extract
  • DNA cloning
  • In vitro recombination
  • Recombinant DNA
  • SLiCE cloning
  • Seamless cloning

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics


Dive into the research topics of 'Seamless Ligation Cloning Extract (SLiCE) cloning method'. Together they form a unique fingerprint.

Cite this