Sea urchin nuclei use rna polymerase ii to transcribe discrete histone RNAs larger than messengers

Shoshana Levy, Geoffrey J. Childs, Laurence Kedes

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

RNA transcribed in isolated sea urchin nuclei and assayed by hybridization to histone genes cloned in E. coil contains sequences homologous to each of the five histone genes. Histone RNA is synthesized exclusively from the same DNA strand which is the template in vivo. Synthesis of the histone gene transcripts is sensitive to α-amanitin concentrations which inhibit RNA polymerase II activity. The fraction of histone RNA synthesized in vitro is comparable at two developmental stages to the fraction synthesized in vivo. The nuclear histone transcripts contain sequences homologous to spacer DNA regions present between the coding regions of the 6500 base pair (bp) histone gene repeat unit. The transcription of spacer sequences was demonstrated by hybridization of the nuclear transcripts to subcloned spacer DNA. Although the bulk of the RNA transcripts are >2000 bases long, the histone-specific transcripts are of discrete sizes ranging from 100 bases to about 1100 bases long. Each histone gene hybridizes with at least one of the larger transcripts and with a different subset of smaller RNAs. We do not detect any giant polycistronic transcript spanning the entire histone repeat unit.

Original languageEnglish (US)
Pages (from-to)151-162
Number of pages12
JournalCell
Volume15
Issue number1
DOIs
StatePublished - 1978
Externally publishedYes

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Sea Urchins
DNA-Directed RNA Polymerases
Histones
RNA
Messenger RNA
Genes
Intergenic DNA
Sequence Homology
Amanitins
RNA Polymerase II
Transcription
Base Pairing

ASJC Scopus subject areas

  • Cell Biology
  • Molecular Biology
  • Medicine(all)

Cite this

Sea urchin nuclei use rna polymerase ii to transcribe discrete histone RNAs larger than messengers. / Levy, Shoshana; Childs, Geoffrey J.; Kedes, Laurence.

In: Cell, Vol. 15, No. 1, 1978, p. 151-162.

Research output: Contribution to journalArticle

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