Screening and Cellular Characterization of Genetically Encoded Voltage Indicators Based on Near-Infrared Fluorescent Proteins

Mikhail V. Monakhov, Mikhail E. Matlashov, Michelangelo Colavita, Chenchen Song, Daria M. Shcherbakova, Srdjan D. Antic, Vladislav V. Verkhusha, Thomas Knöpfel

Research output: Contribution to journalArticlepeer-review

4 Scopus citations

Abstract

We developed genetically encoded voltage indicators using a transmembrane voltage-sensing domain and bright near-infrared fluorescent proteins derived from bacterial phytochromes. These new voltage indicators are excited by 640 nm light and emission is measured at 670 nm, allowing imaging in the near-infrared tissue transparency window. The spectral properties of our new indicators permit seamless voltage imaging with simultaneous blue-green light optogenetic actuator activation as well as simultaneous voltage-calcium imaging when paired with green calcium indicators. Iterative optimizations led to a fluorescent probe, here termed nirButterfly, which reliably reports neuronal activities including subthreshold membrane potential depolarization and hyperpolarization as well as spontaneous spiking or electrically- and optogenetically evoked action potentials. This enables largely improved all-optical causal interrogations of physiology.

Original languageEnglish (US)
Pages (from-to)3523-3531
Number of pages9
JournalACS Chemical Neuroscience
Volume11
Issue number21
DOIs
StatePublished - Nov 4 2020

Keywords

  • all-optical electrophysiology
  • biosensor
  • Butterfly
  • FRET
  • GEVI
  • iRFP

ASJC Scopus subject areas

  • Biochemistry
  • Physiology
  • Cognitive Neuroscience
  • Cell Biology

Fingerprint Dive into the research topics of 'Screening and Cellular Characterization of Genetically Encoded Voltage Indicators Based on Near-Infrared Fluorescent Proteins'. Together they form a unique fingerprint.

Cite this