SCID-hu mice

A model for studying disseminated HIV infection

Harris Goldstein, Massimo Pettoello-Mantovani, Nikos F. Katopodis, Ana Kim, Sergey Yurasov, Tobias R. Kollmann

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Modifications that we introduced into the implantation of human fetal thymus and liver into SCID mice (thy/liv-SCID-hu mice) markedly increased the population of human T cells and monocytes present in the peripheral blood and peripheral lymphoid compartment of these mice. As a result, the modified thy/liv-SCID-hu mice developed disseminated HIV infection after intraimplant or i.p. inoculation. After chronic HIV infection of these mice, depletion of the peripheral human T cells was observed as reported in HIV-infected individuals. In addition, these mice also developed plasma viremia after infection with HIV. The peripheral blood mononuclear cells were responsive to in-vivo cytokine regulation as evidenced by induction of human IFN-γ gene expression by human IL-12 and inhibition by human IL-10. Acute treatment with human IL-10 but not with human IL-12 inhibited the development of plasma viremia and HIV infection in thy/liv-SCID-hu mice inoculated with HIV-159, a clinical isolate. SCID mice transplanted with cultured human fetal bone marrow displayed significant engraftment of the mouse bone marrow with human precursor cells and population of the peripheral blood with human B cells and monocytes. The peripheral blood of these bone marrow-transplanted SCID mice also became populated with human T cells after they were implanted with human thymic tissue due to migration of human precursor cells from the mouse bone marrow to the implanted human thymus. Thus, these modified SCID-hu mice should prove to be a valuable in-vivo model for studying the immunopathogenesis of HIV infection and for examining the in-vivo efficacy of immunomodulatory, drug and gene therapy in modifying HIV infection.

Original languageEnglish (US)
Pages (from-to)223-231
Number of pages9
JournalSeminars in Immunology
Volume8
Issue number4
DOIs
StatePublished - Aug 1996

Fingerprint

SCID Mice
HIV Infections
Bone Marrow
Viremia
Interleukin-12
T-Lymphocytes
Interleukin-10
Thymus Gland
Monocytes
HIV
Immunomodulation
Bone Marrow Cells
Genetic Therapy
Population

Keywords

  • Bone marrow
  • Gene therapy
  • HIV
  • IL-10
  • IL-12
  • SCID mice
  • Thymus
  • Transplantation

ASJC Scopus subject areas

  • Immunology and Allergy
  • Infectious Diseases
  • Immunology

Cite this

Goldstein, H., Pettoello-Mantovani, M., Katopodis, N. F., Kim, A., Yurasov, S., & Kollmann, T. R. (1996). SCID-hu mice: A model for studying disseminated HIV infection. Seminars in Immunology, 8(4), 223-231. https://doi.org/10.1006/smim.1996.0028

SCID-hu mice : A model for studying disseminated HIV infection. / Goldstein, Harris; Pettoello-Mantovani, Massimo; Katopodis, Nikos F.; Kim, Ana; Yurasov, Sergey; Kollmann, Tobias R.

In: Seminars in Immunology, Vol. 8, No. 4, 08.1996, p. 223-231.

Research output: Contribution to journalArticle

Goldstein, H, Pettoello-Mantovani, M, Katopodis, NF, Kim, A, Yurasov, S & Kollmann, TR 1996, 'SCID-hu mice: A model for studying disseminated HIV infection', Seminars in Immunology, vol. 8, no. 4, pp. 223-231. https://doi.org/10.1006/smim.1996.0028
Goldstein H, Pettoello-Mantovani M, Katopodis NF, Kim A, Yurasov S, Kollmann TR. SCID-hu mice: A model for studying disseminated HIV infection. Seminars in Immunology. 1996 Aug;8(4):223-231. https://doi.org/10.1006/smim.1996.0028
Goldstein, Harris ; Pettoello-Mantovani, Massimo ; Katopodis, Nikos F. ; Kim, Ana ; Yurasov, Sergey ; Kollmann, Tobias R. / SCID-hu mice : A model for studying disseminated HIV infection. In: Seminars in Immunology. 1996 ; Vol. 8, No. 4. pp. 223-231.
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