Sca-1pos cells in the mouse mammary gland represent an enriched progenitor cell population

Bryan E. Welm, Stacey B. Tepera, Teresa V. Bowman, Timothy A. Graubert, Jeffrey M. Rosen, Margaret A. Goodell

Research output: Contribution to journalArticle

438 Citations (Scopus)

Abstract

Mammary epithelium can functionally regenerate upon transplantation. This renewal capacity has been classically ascribed to the function of a multipotent mammary gland stem cell population, which has been hypothesized to be a primary target in the etiology of breast cancer. Several complementary approaches were employed in this study to identify and enrich mammary epithelial cells that retain stem cell characteristics. Using long-term BrdU labeling, a population of label retaining cells (LRCs) that lack expression of differentiation markers has been identified. LRCs isolated from mammary primary cultures were enriched for stem cell antigen-1 (Sca-1) and Hoechst dye-effluxing "side population" properties. Sca-1pos cells in the mammary gland were localized to the luminal epithelia by using Sca-1+/GFP mice, were progesterone receptor-negative, and did not bind peanut lectin. Finally, the Sca-1pos population is enriched for functional stem/progenitor cells, as demonstrated by its increased regenerative potential compared with Sca-1neg cells when transplanted into the cleared mammary fat pads of host mice.

Original languageEnglish (US)
Pages (from-to)42-56
Number of pages15
JournalDevelopmental Biology
Volume245
Issue number1
DOIs
StatePublished - May 1 2002
Externally publishedYes

Fingerprint

Human Mammary Glands
Stem Cells
Breast
Population
Epithelium
Peanut Agglutinin
Differentiation Antigens
Progesterone Receptors
Bromodeoxyuridine
Adipose Tissue
Coloring Agents
Transplantation
Epithelial Cells
Breast Neoplasms
Antigens

Keywords

  • BrdU
  • Label retention
  • Mammary
  • Progesterone receptor
  • Sca-1
  • Stem cells

ASJC Scopus subject areas

  • Developmental Biology

Cite this

Sca-1pos cells in the mouse mammary gland represent an enriched progenitor cell population. / Welm, Bryan E.; Tepera, Stacey B.; Bowman, Teresa V.; Graubert, Timothy A.; Rosen, Jeffrey M.; Goodell, Margaret A.

In: Developmental Biology, Vol. 245, No. 1, 01.05.2002, p. 42-56.

Research output: Contribution to journalArticle

Welm, Bryan E. ; Tepera, Stacey B. ; Bowman, Teresa V. ; Graubert, Timothy A. ; Rosen, Jeffrey M. ; Goodell, Margaret A. / Sca-1pos cells in the mouse mammary gland represent an enriched progenitor cell population. In: Developmental Biology. 2002 ; Vol. 245, No. 1. pp. 42-56.
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