Sca-1pos cells in the mouse mammary gland represent an enriched progenitor cell population

Bryan E. Welm, Stacey B. Tepera, Teresa Venezia, Timothy A. Graubert, Jeffrey M. Rosen, Margaret A. Goodell

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439 Scopus citations

Abstract

Mammary epithelium can functionally regenerate upon transplantation. This renewal capacity has been classically ascribed to the function of a multipotent mammary gland stem cell population, which has been hypothesized to be a primary target in the etiology of breast cancer. Several complementary approaches were employed in this study to identify and enrich mammary epithelial cells that retain stem cell characteristics. Using long-term BrdU labeling, a population of label retaining cells (LRCs) that lack expression of differentiation markers has been identified. LRCs isolated from mammary primary cultures were enriched for stem cell antigen-1 (Sca-1) and Hoechst dye-effluxing "side population" properties. Sca-1pos cells in the mammary gland were localized to the luminal epithelia by using Sca-1+/GFP mice, were progesterone receptor-negative, and did not bind peanut lectin. Finally, the Sca-1pos population is enriched for functional stem/progenitor cells, as demonstrated by its increased regenerative potential compared with Sca-1neg cells when transplanted into the cleared mammary fat pads of host mice.

Original languageEnglish (US)
Pages (from-to)42-56
Number of pages15
JournalDevelopmental Biology
Volume245
Issue number1
DOIs
StatePublished - May 1 2002

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Keywords

  • BrdU
  • Label retention
  • Mammary
  • Progesterone receptor
  • Sca-1
  • Stem cells

ASJC Scopus subject areas

  • Molecular Biology
  • Developmental Biology
  • Cell Biology

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