@article{adca8580b5a64079bbc31d8d9cc5631a,
title = "Satb1 regulates the self-renewal of hematopoietic stem cells by promoting quiescence and repressing differentiation commitment",
abstract = "How hematopoietic stem cells (HSCs) coordinate the regulation of opposing cellular mechanisms such as self-renewal and differentiation commitment remains unclear. Here we identified the transcription factor and chromatin remodeler Satb1 as a critical regulator of HSC fate. HSCs lacking Satb1 had defective self-renewal, were less quiescent and showed accelerated lineage commitment, which resulted in progressive depletion of functional HSCs. The enhanced commitment was caused by less symmetric self-renewal and more symmetric differentiation divisions of Satb1-deficient HSCs. Satb1 simultaneously repressed sets of genes encoding molecules involved in HSC activation and cellular polarity, including Numb and Myc, which encode two key factors for the specification of stem-cell fate. Thus, Satb1 is a regulator that promotes HSC quiescence and represses lineage commitment.",
author = "Britta Will and Vogler, {Thomas O.} and Boris Bartholdy and Francine Garrett-Bakelman and Jillian Mayer and Laura Barreyro and Ashley Pandolfi and Todorova, {Tihomira I.} and Okoye-Okafor, {Ujunwa C.} and Stanley, {Robert F.} and Bhagat, {Tushar D.} and Amit Verma and Figueroa, {Maria E.} and Ari Melnick and Michael Roth and Ulrich Steidl",
note = "Funding Information: We thank T. Kohwi-Shigematsu (Lawrence Berkeley National Laboratory) for Satb1−/− mice; J. Bradner (Dana-Farber Cancer Institute, Harvard Medical School) for JQ1; T. Kohwi-Shigematsu, E. Passegu{\'e}, M. Alberich-Jord{\'a} and the members of the Steidl laboratory for discussions and suggestions; G. Simkin, and S. Narayanagari of the Einstein Human Stem Cell FACS and Xenotransplantation Facility; and P. Schultes, C. Sheridan and the Epigenomics Core Facility of Weill Cornell Medical College for technical assistance. Supported by the American Cancer Society (121366-PF-12-89-01-TBG to B.W.), the Sass Foundation (F.G.B.), the National Cancer Institute (1K08CA169055-01 to F.G.B. and R00CA131503 to U.S.), the US National Institutes of Health (F31CA162770 to U.C.O.-O. and F30HL117545 to A.P.) and New York State Stem Cell Science (C024306, C026416 and C028116 to U.S., and C024172 to E. Bouhassira). U.S. is the Diane and Arthur B. Belfer Faculty Scholar in Cancer Research of the Albert Einstein College of Medicine.",
year = "2013",
month = may,
doi = "10.1038/ni.2572",
language = "English (US)",
volume = "14",
pages = "437--445",
journal = "Nature Immunology",
issn = "1529-2908",
publisher = "Nature Publishing Group",
number = "5",
}