Satb1 is an activity-modulated transcription factor required for the terminal differentiation and connectivity of medial ganglionic eminence-derived cortical interneurons

Jennie Close, Han Xu, Natalia De Marco García, Renata A. Batista-Brito, Elsa Rossignol, Bernardo Rudy, Gord Fishell

Research output: Contribution to journalArticle

63 Citations (Scopus)

Abstract

Although previous work identified transcription factors crucial for the specification and migration of parvalbumin (PV)-expressing and somatostatin (SST)-expressing interneurons, the intrinsic factors required for the terminal differentiation, connectivity, and survival of these cell types remain uncharacterized. Here we demonstrate that, with in subpopulations of cortical interneurons, Satb 1 (special AT-rich binding protein) promotes terminal differentiation, connectivity, and survival in interneurons that express PV and SST. Wefind that conditionalremovalof Satb1 in mouse interneurons results in the loss of a majority of SST-expressing cells across all cortical layers, as well as some PV-expressing cells in layers IV and VI, by postnatal day 21. SST-expressing cells initially migrate to the cortex in Satb1 mutant mice, but receive reduced levels of afferent input and begin to die during the first postnatal week. Electrophysiological characterization indicates that loss of Satb1 function in interneurons results in a loss of functional inhibition of excitatory principal cells. These data suggest that Satb1 is required for medial ganglionic eminence-derived interneuron differentiation, connectivity, and survival.

Original languageEnglish (US)
Pages (from-to)17690-17705
Number of pages16
JournalJournal of Neuroscience
Volume32
Issue number49
DOIs
StatePublished - Dec 5 2012
Externally publishedYes

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Median Eminence
Interneurons
Transcription Factors
Parvalbumins
Somatostatin-Secreting Cells
Somatostatin
Intrinsic Factor
Cell Survival
Carrier Proteins

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Satb1 is an activity-modulated transcription factor required for the terminal differentiation and connectivity of medial ganglionic eminence-derived cortical interneurons. / Close, Jennie; Xu, Han; García, Natalia De Marco; Batista-Brito, Renata A.; Rossignol, Elsa; Rudy, Bernardo; Fishell, Gord.

In: Journal of Neuroscience, Vol. 32, No. 49, 05.12.2012, p. 17690-17705.

Research output: Contribution to journalArticle

Close, Jennie ; Xu, Han ; García, Natalia De Marco ; Batista-Brito, Renata A. ; Rossignol, Elsa ; Rudy, Bernardo ; Fishell, Gord. / Satb1 is an activity-modulated transcription factor required for the terminal differentiation and connectivity of medial ganglionic eminence-derived cortical interneurons. In: Journal of Neuroscience. 2012 ; Vol. 32, No. 49. pp. 17690-17705.
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