Sarcoglycan complex. Implications for metabolic defects in muscular dystrophies

Séverine Groh, Haihong Zong, Matthew M. Goddeeris, Connie S. Lebakken, David Venzke, Jeffrey E. Pessin, Kevin P. Campbell

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

The sarcoglycans are known as an integral subcomplex of the dystrophin glycoprotein complex, the function of which is best characterized in skeletal muscle in relation to muscular dystrophies. Here we demonstrate that the white adipocytes, which share a common precursor with the myocytes, express a cellspecific sarcoglycan complex containing β-, δ-, and ∈-sarcoglycan. In addition, the adipose sarcoglycan complex associates with sarcospan and laminin binding dystroglycan. Using multiple sarcoglycan null mouse models, we show that loss of α-sarcoglycan has no consequence on the expression of the adipocyte sarcoglycan complex. However, loss of β- or δ-sarcoglycan leads to a concomitant loss of the sarcoglycan complex as well as sarcospan and a dramatic reduction in dystroglycan in adipocytes. We further demonstrate that β-sarcoglycan null mice, which lack the sarcoglycan complex in adipose tissue and skeletal muscle, are glucose-intolerant and exhibit whole body insulin resistance specifically due to impaired insulin-stimulated glucose uptake in skeletal muscles. Thus, our data demonstrate a novel function of the sarcoglycan complex in whole body glucose homeostasis and skeletal muscle metabolism, suggesting that the impairment of the skeletal muscle metabolism influences the pathogenesis of muscular dystrophy.

Original languageEnglish (US)
Pages (from-to)19178-19182
Number of pages5
JournalJournal of Biological Chemistry
Volume284
Issue number29
DOIs
StatePublished - Jul 17 2009

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Sarcoglycans
Muscular Dystrophies
Defects
Muscle
Skeletal Muscle
Dystroglycans
Adipocytes
Metabolism
Glucose
White Adipocytes
Insulin
Dystrophin
Laminin
Muscle Cells

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology
  • Molecular Biology

Cite this

Sarcoglycan complex. Implications for metabolic defects in muscular dystrophies. / Groh, Séverine; Zong, Haihong; Goddeeris, Matthew M.; Lebakken, Connie S.; Venzke, David; Pessin, Jeffrey E.; Campbell, Kevin P.

In: Journal of Biological Chemistry, Vol. 284, No. 29, 17.07.2009, p. 19178-19182.

Research output: Contribution to journalArticle

Groh, Séverine ; Zong, Haihong ; Goddeeris, Matthew M. ; Lebakken, Connie S. ; Venzke, David ; Pessin, Jeffrey E. ; Campbell, Kevin P. / Sarcoglycan complex. Implications for metabolic defects in muscular dystrophies. In: Journal of Biological Chemistry. 2009 ; Vol. 284, No. 29. pp. 19178-19182.
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