Sampling Hyperpolarized Molecules Utilizing a 1 Tesla Permanent Magnetic Field

Sui Seng Tee; Valentina DiGialleonardo; Roozbeh Eskandari; Sangmoo Jeong; Kristin L. Granlund; Vesselin Miloushev; Alex J. Poot; Steven Truong; Julio A. Alvarez; Hannah N. Aldeborgh; Kayvan R. Keshari

doi:10.1038/srep32846

Sampling Hyperpolarized Molecules Utilizing a 1 Tesla Permanent Magnetic Field

Sui Seng Tee, Valentina DiGialleonardo, Roozbeh Eskandari, Sangmoo Jeong, Kristin L. Granlund, Vesselin Miloushev, Alex J. Poot, Steven Truong, Julio A. Alvarez, Hannah N. Aldeborgh, Kayvan R. Keshari

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Abstract

Hyperpolarized magnetic resonance spectroscopy (HP MRS) using dynamic nuclear polarization (DNP) is a technique that has greatly enhanced the sensitivity of detecting ¹³C nuclei. However, the HP MRS polarization decays in the liquid state according to the spin-lattice relaxation time (T₁) of the nucleus. Sampling of the signal also destroys polarization, resulting in a limited temporal ability to observe biologically interesting reactions. In this study, we demonstrate that sampling hyperpolarized signals using a permanent magnet at 1 Tesla (1T) is a simple and cost-effective method to increase T₁s without sacrificing signal-to-noise. Biologically-relevant information may be obtained with a permanent magnet using enzyme solutions and in whole cells. Of significance, our findings indicate that changes in pyruvate metabolism can also be quantified in a xenograft model at this field strength.

Original language	English (US)
Article number	32846
Journal	Scientific reports
Volume	6
DOIs	https://doi.org/10.1038/srep32846
State	Published - Sep 6 2016
Externally published	Yes

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title = "Sampling Hyperpolarized Molecules Utilizing a 1 Tesla Permanent Magnetic Field",

abstract = "Hyperpolarized magnetic resonance spectroscopy (HP MRS) using dynamic nuclear polarization (DNP) is a technique that has greatly enhanced the sensitivity of detecting 13C nuclei. However, the HP MRS polarization decays in the liquid state according to the spin-lattice relaxation time (T1) of the nucleus. Sampling of the signal also destroys polarization, resulting in a limited temporal ability to observe biologically interesting reactions. In this study, we demonstrate that sampling hyperpolarized signals using a permanent magnet at 1 Tesla (1T) is a simple and cost-effective method to increase T1s without sacrificing signal-to-noise. Biologically-relevant information may be obtained with a permanent magnet using enzyme solutions and in whole cells. Of significance, our findings indicate that changes in pyruvate metabolism can also be quantified in a xenograft model at this field strength.",

author = "Tee, {Sui Seng} and Valentina DiGialleonardo and Roozbeh Eskandari and Sangmoo Jeong and Granlund, {Kristin L.} and Vesselin Miloushev and Poot, {Alex J.} and Steven Truong and Alvarez, {Julio A.} and Aldeborgh, {Hannah N.} and Keshari, {Kayvan R.}",

note = "Publisher Copyright: {\textcopyright} The Author(s) 2016.",

year = "2016",

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doi = "10.1038/srep32846",

language = "English (US)",

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T1 - Sampling Hyperpolarized Molecules Utilizing a 1 Tesla Permanent Magnetic Field

AU - Tee, Sui Seng

AU - DiGialleonardo, Valentina

AU - Eskandari, Roozbeh

AU - Jeong, Sangmoo

AU - Granlund, Kristin L.

AU - Miloushev, Vesselin

AU - Poot, Alex J.

AU - Truong, Steven

AU - Alvarez, Julio A.

AU - Aldeborgh, Hannah N.

AU - Keshari, Kayvan R.

PY - 2016/9/6

Y1 - 2016/9/6

N2 - Hyperpolarized magnetic resonance spectroscopy (HP MRS) using dynamic nuclear polarization (DNP) is a technique that has greatly enhanced the sensitivity of detecting 13C nuclei. However, the HP MRS polarization decays in the liquid state according to the spin-lattice relaxation time (T1) of the nucleus. Sampling of the signal also destroys polarization, resulting in a limited temporal ability to observe biologically interesting reactions. In this study, we demonstrate that sampling hyperpolarized signals using a permanent magnet at 1 Tesla (1T) is a simple and cost-effective method to increase T1s without sacrificing signal-to-noise. Biologically-relevant information may be obtained with a permanent magnet using enzyme solutions and in whole cells. Of significance, our findings indicate that changes in pyruvate metabolism can also be quantified in a xenograft model at this field strength.

AB - Hyperpolarized magnetic resonance spectroscopy (HP MRS) using dynamic nuclear polarization (DNP) is a technique that has greatly enhanced the sensitivity of detecting 13C nuclei. However, the HP MRS polarization decays in the liquid state according to the spin-lattice relaxation time (T1) of the nucleus. Sampling of the signal also destroys polarization, resulting in a limited temporal ability to observe biologically interesting reactions. In this study, we demonstrate that sampling hyperpolarized signals using a permanent magnet at 1 Tesla (1T) is a simple and cost-effective method to increase T1s without sacrificing signal-to-noise. Biologically-relevant information may be obtained with a permanent magnet using enzyme solutions and in whole cells. Of significance, our findings indicate that changes in pyruvate metabolism can also be quantified in a xenograft model at this field strength.

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Sampling Hyperpolarized Molecules Utilizing a 1 Tesla Permanent Magnetic Field

Abstract

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