S100A4 regulates macrophage chemotaxis

Zhong Hua Li, Natalia Dulianinova, Reniqua P. House, Steven C. Almo, Anne R. Bresnick

Research output: Contribution to journalArticle

66 Citations (Scopus)

Abstract

S100A4, a member of the S100 family of Ca2+-binding proteins, is directly involved in tumor metastasis. In addition to its expression in tumor cells, S100A4 is expressed in normal cells and tissues, including fibroblasts and cells of the immune system. To examine the contribution of S100A4 to normal physiology, we established S100A4-deficient mice by gene targeting. Homozygous S100A4-/- mice are fertile, grow normally and exhibit no overt abnormalities; however, the loss of S100A4 results in impaired recruitment of macrophages to sites of inflammation in vivo. Consistent with these observations, primary bone marrow macrophages (BMMs) derived from S100A4 -/- mice display defects in chemotactic motility in vitro. S100A4-/- BMMs form unstable protrusions, overassemble myosin-IIA, and exhibit altered colony-stimulating factor-1 receptor signaling. These studies establish S100A4 as a regulator of physiological macrophage motility and demonstrate that S100A4 mediates macrophage recruitment and chemotaxis in vivo.

Original languageEnglish (US)
Pages (from-to)2598-2610
Number of pages13
JournalMolecular Biology of the Cell
Volume21
Issue number15
DOIs
StatePublished - Aug 1 2010

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Chemotaxis
Macrophages
Nonmuscle Myosin Type IIA
Colony-Stimulating Factor Receptors
Macrophage Colony-Stimulating Factor
Gene Targeting
Immune System
Neoplasms
Carrier Proteins
Fibroblasts
Bone Marrow
Neoplasm Metastasis
Inflammation

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

Cite this

S100A4 regulates macrophage chemotaxis. / Li, Zhong Hua; Dulianinova, Natalia; House, Reniqua P.; Almo, Steven C.; Bresnick, Anne R.

In: Molecular Biology of the Cell, Vol. 21, No. 15, 01.08.2010, p. 2598-2610.

Research output: Contribution to journalArticle

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