S-layer proteins of Lactobacillus acidophilus inhibits JUNV infection

María Guadalupe Martínez, Mariano Prado Acosta, Nélida A. Candurra, Sandra M. Ruzal

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

It has been previously described that S-layer binds to the C-type lectin DC-specific ICAM-3-grabbing nonintegrin (DC-SIGN, CD209). It was also shown that DC-SIGN is a cell-surface adhesion factor that enhances viral entry of several virus families. Among those, Junin virus (JUNV) entry is enhanced in cells expressing DC-SIGN and for that reason surface-layer protein (S-layer) of Lactobacillus acidophilus ATCC 4365 was evaluated as a possible JUNV inhibitor. Experiments using 3T3 cells stably expressing DC-SIGN, showed an almost complete inhibition of JUNV infection when they were treated with S-layer in a similar extend as the inhibition shown by mannan. However no inhibition effect was observed in 3T3 wild type cells or in 3T3 cells expressing liver/lymph node-specific ICAM-3 grabbing nonintegrin (L-SIGN or DC-SIGNR or CD209L).Treatments with S-layer during different times in the infection demonstrated that inhibition was only observed when S-layer was presented in early stages of the viral infection. This inhibition does not involve the classic recognition of mannose by this C-type lectin as the S-layer showed no evidence to be glycosylated. In fact, the highly basic nature of the S-layer (pI>9.5) seems to be involved in electrostatic interactions between DC-SIGN and S-layer, since high pH abolished the inhibitory effect on infection cause by the S-layer. In silico analysis predicts a Ca 2+-dependant carbohydrate recognition domain in the SlpA protein.This novel characteristic of the S-layer, a GRAS status protein, contribute to the pathogen exclusion reported for this probiotic strain and may be applied as an antiviral agent to inhibit several kinds of viruses.

Original languageEnglish (US)
Pages (from-to)590-595
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume422
Issue number4
DOIs
StatePublished - Jun 15 2012
Externally publishedYes

Fingerprint

Junin virus
Lactobacillus acidophilus
Virus Diseases
Viruses
Proteins
3T3 Cells
C-Type Lectins
Virus Internalization
Cells
S-layer proteins
Mannans
Probiotics
Pathogens
Mannose
Infection
Coulomb interactions
DC-specific ICAM-3 grabbing nonintegrin
Static Electricity
Liver
Computer Simulation

Keywords

  • Antiviral
  • Lactobacillus
  • Surface layer

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Cell Biology
  • Molecular Biology

Cite this

S-layer proteins of Lactobacillus acidophilus inhibits JUNV infection. / Martínez, María Guadalupe; Prado Acosta, Mariano; Candurra, Nélida A.; Ruzal, Sandra M.

In: Biochemical and Biophysical Research Communications, Vol. 422, No. 4, 15.06.2012, p. 590-595.

Research output: Contribution to journalArticle

Martínez, María Guadalupe ; Prado Acosta, Mariano ; Candurra, Nélida A. ; Ruzal, Sandra M. / S-layer proteins of Lactobacillus acidophilus inhibits JUNV infection. In: Biochemical and Biophysical Research Communications. 2012 ; Vol. 422, No. 4. pp. 590-595.
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