S-allylcysteine induces cytotoxic effects in two human lung cancer cell lines via induction of oxidative damage, downregulation of Nrf2 and NF-κB, and apoptosis

Mario Orozco-Morales, Norma Yanet Hernández-Pedro, Pedro Barrios-Bernal, Oscar Arrieta, Luz María Ruiz-Godoy, Michael Aschner, Abel Santamaría, Ana Laura Colín-González

Research output: Contribution to journalArticlepeer-review

Abstract

In this study, we investigated the putative cytotoxic effect elicited by the garlic-derived compound S-allylcysteine (SAC) in two human cancer cell lines (HCC827 and NCIH1975) in order to develop an experimental approach to the therapeutic potential of this molecule for lung cancer. Cells were incubated for 24, 48 and 72 h in the presence of SAC (10 or 20 mM), which resulted in a concentrationand time-dependent decrease in cell viability and culture confluence in both cell lines. These effects were contrasted with - and validated through - those observed in an immortalized but nontumorigenic epithelial cell line from human bronchial epithelium (BEAS-2B, negative control) and an adenocarcinoma human alveolar basal epithelial cell line (A549, positive control). SAC (20 mM at 72 h) also increased the oxidative damage to lipids, augmented apoptosis, and decreased the expression of the nuclear factor erythroid 2-related factor 2 (Nrf2) and the nuclear factor kappa B (NF-κB) proteins in HCC827 and NCI-H1975 cells. Our results establish the efficacy of SAC in reducing malignant growth and proliferation of lung tumor cells. This effect is mediated by the induction of oxidative damage associated with the downregulation of Nrf2 and NF-κB and their corresponding signaling pathways. Anti-Cancer Drugs 32: 117-126.

Original languageEnglish (US)
Pages (from-to)117-126
Number of pages10
JournalAnti-Cancer Drugs
DOIs
StatePublished - Feb 1 2021

Keywords

  • Antineoplastic properties
  • Apoptotic cell death
  • Cytotoxicity
  • Garlic-derived compound
  • Lung cancer cell lines

ASJC Scopus subject areas

  • Oncology
  • Pharmacology
  • Pharmacology (medical)
  • Cancer Research

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