RUNX3 protein is overexpressed in human epithelial ovarian cancer

Nicole S. Nevadunsky, John S. Barbieri, Joseph Kwong, Melissa A. Merritt, William R. Welch, Ross S. Berkowitz, Samuel C. Mok

Research output: Contribution to journalArticle

35 Scopus citations

Abstract

Objective: RUNX family genes, including RUNX3, are developmental regulators that are important in human cancers. The purpose of this study was to evaluate expression and oncogenic potential of RUNX3 in ovarian carcinoma. Methods: Immunohistochemical staining was performed on 60 malignant, 14 borderline, and 5 normal ovarian specimens. Correlation between RUNX3 expression with tumor histology was performed. RUNX3 expression was evaluated by quantitative real-time polymerase chain reaction (QRT-PCR) in microdissected normal and malignant epithelial ovarian tissues. Cell proliferation and viability studies were performed on cells expressing RUNX3 by lentiviral infection and cells with silenced RUNX3 expression by siRNA. Results: RUNX3 expression by immunohistochemistry was higher in serous ovarian carcinomas versus normal ovarian epithelium (P < 0.001). Immunofluorescent staining confirmed upregulation of cytoplasmic RUNX3 in ovarian cancer cell lines and tissues. QRT-PCR showed higher RUNX3 mRNA expression in microdissected borderline and malignant ovarian tumor tissues compared with the normal ovarian surface epithelial cells (HOSE) (P = 0.006 and P = 0.023). Forced RUNX3 expression by lentiviral gene delivery in ovarian cancer cells, SKOV3, that initially showed undetectable RUNX3 expression, resulted in increased cell viability (P = 0.043). Silencing RUNX3 expression by siRNA transfection into ovarian cancer cells, OVCAR429, initially expressing high levels of endogenous RUNX3 resulted in a decrease in proliferation (P = 0.021). Conclusion: These results suggest that RUNX3 has a role in cell proliferation and viability in ovarian cancer.

Original languageEnglish (US)
Pages (from-to)325-330
Number of pages6
JournalGynecologic Oncology
Volume112
Issue number2
DOIs
StatePublished - Feb 1 2009
Externally publishedYes

Keywords

  • Oncogene
  • Ovarian cancer
  • RUNX3

ASJC Scopus subject areas

  • Oncology
  • Obstetrics and Gynecology

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    Nevadunsky, N. S., Barbieri, J. S., Kwong, J., Merritt, M. A., Welch, W. R., Berkowitz, R. S., & Mok, S. C. (2009). RUNX3 protein is overexpressed in human epithelial ovarian cancer. Gynecologic Oncology, 112(2), 325-330. https://doi.org/10.1016/j.ygyno.2008.09.006