TY - JOUR
T1 - Rotavirus RNAs sponge host cell RNA binding proteins and interfere with their subcellular localization
AU - Oceguera, Alfonso
AU - Peralta, Andrea V.
AU - Martínez-Delgado, Gustavo
AU - Arias, Carlos F.
AU - López, Susana
N1 - Publisher Copyright:
© 2018 Elsevier Inc.
PY - 2018/12
Y1 - 2018/12
N2 - Cellular mRNAs cycle between translating and non-translating pools, polysomes compose the translating pool, while RNA granules contain translationally-silenced mRNAs, where the RNAs are either stored in stress granules, or accumulate in processing bodies (PBs) or GW-bodies, which have an important role in RNA degradation. Viruses have developed measures to prevent the deleterious effects of these structures during their replication. Rotavirus, the most common agent of viral gastroenteritis, is capable of establishing a successful infection by counteracting several of the antiviral responses of its host. Here, we describe that in rotavirus-infected cells the distribution of several RNA binding proteins is changed causing the disaggregation of PBs, the relocalization of GW-body proteins, and the cytoplasmic accumulation of HuR, a predominantly nuclear protein. We show that this redistribution of proteins is more likely caused by the accumulation of viral RNA in the cytoplasm of infected-cells, where it might be acting as an RBP sponge.
AB - Cellular mRNAs cycle between translating and non-translating pools, polysomes compose the translating pool, while RNA granules contain translationally-silenced mRNAs, where the RNAs are either stored in stress granules, or accumulate in processing bodies (PBs) or GW-bodies, which have an important role in RNA degradation. Viruses have developed measures to prevent the deleterious effects of these structures during their replication. Rotavirus, the most common agent of viral gastroenteritis, is capable of establishing a successful infection by counteracting several of the antiviral responses of its host. Here, we describe that in rotavirus-infected cells the distribution of several RNA binding proteins is changed causing the disaggregation of PBs, the relocalization of GW-body proteins, and the cytoplasmic accumulation of HuR, a predominantly nuclear protein. We show that this redistribution of proteins is more likely caused by the accumulation of viral RNA in the cytoplasm of infected-cells, where it might be acting as an RBP sponge.
KW - GW-bodies
KW - P-bodies
KW - RNA binding proteins
KW - RNA granules
KW - RNA sponges
KW - Rotavirus
KW - Virus host cell interactions
UR - http://www.scopus.com/inward/record.url?scp=85053849042&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85053849042&partnerID=8YFLogxK
U2 - 10.1016/j.virol.2018.09.013
DO - 10.1016/j.virol.2018.09.013
M3 - Article
C2 - 30253276
AN - SCOPUS:85053849042
SN - 0042-6822
VL - 525
SP - 96
EP - 105
JO - Virology
JF - Virology
ER -